Pulsed-field gel electrophoresis to study the diversity of whole-genome organization in the genus Ochrobactrum

被引：20

作者：

Teyssier, C

Marchandin, H

Masnou, A

Jeannot, JL

de Buochberg, MS

Jumas-Bilak, E

机构：

[1] Fac Pharm Montpellier, Bacteriol Lab, F-34093 Montpellier, France

[2] Hop Arnaud Villeneuve, Bacteriol Lab, Montpellier, France

来源：

ELECTROPHORESIS | 2005年 / 26卷 / 15期

关键词：

genome; I-Ceul; Ochrobactrurn; polymorphism; pulsed-field gel electrophoresis;

D O I：

10.1002/elps.200410323

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The alpha-proteobacterial genus Ochrobactrum groups together organisms that display varied life-styles, such as free-living bacteria, members of rhizosphere and soil, nitrogen-fixing bacteria in plant nodules, xenobiotic-degrading bacteria, colonizers of nematodes and insects, and opportunistic human pathogens. The genomes of nine strains of Ochrobactrum anthropi and eight strains of Ochrobactrum, intermedium were analyzed by pulsed-field gel electrophoresis of the whole genome and of I-Ceul digestion fragments. All isolates and type strains of O. anthropi and O. intermedium possessed two high-molecular-weight circular replicons identified as two independent chromosomes on the basis of 16S rDNA hybridization. The genome of the type strain of Ochrobactrum tritici, Ochrobactrum grignonense, and Ochrobactrum gallinifaecis also contained two circular chromosomes. The megaplasmid content was highly variable even among strains in the same species, leading to whole-genome sizes that ranged from 5.060 to 8.300 Mbp and from 4.690 to 7.680 Mbp for O. anthropi and O. intermedium, respectively. This exceptional level of genomic diversity could be related to the adaptability of Ochrobactrum spp. to various ecological niches.

引用

页码：2898 / 2907

页数：10

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