Impaired Biomechanical Properties of Diabetic Skin Implications in Pathogenesis of Diabetic Wound Complications

Diabetic skin is known to have deficient wound healing properties, but little is known of its intrinsic biomeclhanical properties. We hypothesize that diabetic skin possesses inferior biomechanical properties at baseline, rendering it more prone to injury. Skin from diabetic and nondiabetic mice and humans underwent biomechanical testing. Real-time PCR was performed for genes integral to collagen synthesis and degradation. MMP-2 and MMP-9, and TEMP-1 protein levels were assessed by ELISA and zymography. Collagen I and RI content was assessed using Western blot analysis. At baseline, both murine and human diabetic skin was biomechanically inferior compared to nondiabetic skin, with decreased maximum stress and decreased modulus (P < 0.001 and < 0.05, respectively). Surprisingly, the expression of genes involved in collagen synthesis were significantly up-regulated, and genes involved in collagen degradation were significantly down-regulated In murine diabetic skin (P < 0.01). In addition, MMP-2 and MMP-9/TBIP-1 protein ratios were significantly lower in murine diabetic skin (P < 0.05). Collagen I levels and I:M ratios were lower in diabetic skin (P < 0.05). These findings suggest that the predisposition of diabetics to wounds may be the result of impaired tissue integrity at baseline, and are due, in part, to a defect in the regulation of collagen protein synthesis at the post-transcriptional level. (AmJ Pathol 2011, 178: 2215-2223; DOI: 10.1016/j.ajpath.2011.01.015)