Synthesis and characterization of a novel near-infrared fluorescent probe for applications in imaging A549 cells

被引：3

作者：

Xing, Jing ^{[1
,2
,3
]}

Zhou, Gaoxin ^{[1
,2
,3
]}

Sun, Chunlong ^{[4
]}

Zhang, Huanqing ^{[5
]}

Chen, Bo ^{[1
,2
,3
]}

Zong, Xi ^{[6
]}

Cai, Jin ^{[6
]}

Ji, Min ^{[1
,2
,3
]}

机构：

[1] Southeast Univ, Sch Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China

[2] Southeast Univ, Sch Biol Sci & Med Engn, Suzhou 215123, Peoples R China

[3] Southeast Univ, Collaborat Innovat Ctr Suzhou Nano Sci & Technol, Suzhou 215123, Peoples R China

[4] Binzhou Univ, Coll Life Sci, Binzhou 256600, Peoples R China

[5] Chia Tai Tianqing Pharmaceut Grp Co Ltd, 699-8 Xuanwu Blvd, Nanjing 210042, Jiangsu, Peoples R China

[6] Southeast Univ, Sch Chem & Chem Engn, Nanjing 210096, Jiangsu, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2016年 / 38卷 / 11期

关键词：

Fluorescent dye; Fluorescence imaging; IR-793; Imaging of living cells; Indocyanine Green; Living cells; A549; cells; CANCER;

D O I：

10.1007/s10529-016-2179-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To design and synthesize a novel near-infrared (NIR) fluorescent probe based on indocyanine Green (ICG), that can be applied in imaging living cells. A highly fluorescent novel NIR fluorescent probe (IR-793) was synthesized in two steps. IR-793 had better fluorescence and optical stability than ICG. In addition, no obvious cytotoxicity effect of IR-793 was observed and cell viability was above 75% at the maximum concentration (120 nM). IR-793 also exhibited good performance in imaging living A549 cells. IR-793, a novel NIR fluorescent probe that is stable, low-cost, highly fluorescent and low cytotoxicity, has been designed and synthesized for imaging living cells.

引用

页码：1851 / 1856

页数：6

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