Monomeric G-protein-coupled receptor as a functional unit

被引:166
作者
Chabre, M
le Maire, M
机构
[1] CNRS, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
[2] Univ Nice Sophia Antipolis, F-06560 Valbonne, France
[3] Univ Paris 11, CNRS, Dept Biol Joliot Curie, F-91191 Gif Sur Yvette, France
[4] CEA Saclay, F-91191 Gif Sur Yvette, France
关键词
D O I
10.1021/bi050720o
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Rhodopsin, the first purified G-protein-coupled receptor (GPCR), was characterized as a functional monomer 30 year ago, but dimerization of GPCRs recently became the new paradigm of signal transduction. It has even been claimed, on the basis of recent biophysical and biochemical studies, that this new concept could be extended to higher-order oligomerization. Here this view is challenged. The new studies of rhodopsin and other simple (class 1a) GPCRs solubilized in detergent are re-assessed and are compared to the earlier classical studies of rhodopsin and other membrane proteins solubilized in detergent. The new studies are found to strengthen rather than invalidate the conclusions of the early ones and to support a monomeric model for rhodopsin and other class 1a GPCRs. A molecular model is proposed for the functional coupling of a rhodopsin monomeric unit with a G-protein heterotrimer. This model should be valid even for GPCRs that exist as structural dimers.
引用
收藏
页码:9395 / 9403
页数:9
相关论文
共 68 条
[1]   Structural features and light-dependent changes in the cytoplasmic interhelical E-F loop region of rhodopsin: A site-directed spin-labeling study [J].
Altenbach, C ;
Yang, K ;
Farrens, DL ;
Farahbakhsh, ZT ;
Khorana, HG ;
Hubbell, WL .
BIOCHEMISTRY, 1996, 35 (38) :12470-12478
[2]   Structure-based analysis of GPCR function:: Evidence for a novel pentameric assembly between the dimeric leukotriene B4 receptor BLT1 and the G-protein [J].
Banères, JL ;
Parello, J .
JOURNAL OF MOLECULAR BIOLOGY, 2003, 329 (04) :815-829
[3]   Cell-free synthesis of a functional ion channel in the absence of a membrane and in the presence of detergent [J].
Berrier, C ;
Park, KH ;
Abes, S ;
Bibonne, A ;
Betton, JM ;
Ghazi, A .
BIOCHEMISTRY, 2004, 43 (39) :12585-12591
[4]   ROLES OF LIPID MODIFICATIONS OF TRANSDUCIN SUBUNITS IN THEIR GDP-DEPENDENT ASSOCIATION AND MEMBRANE-BINDING [J].
BIGAY, J ;
FAUROBERT, E ;
FRANCO, M ;
CHABRE, M .
BIOCHEMISTRY, 1994, 33 (47) :14081-14090
[5]   Molecular tinkering of G protein-coupled receptors: an evolutionary success [J].
Bockaert, J ;
Pin, JP .
EMBO JOURNAL, 1999, 18 (07) :1723-1729
[6]   THE TRANSITORY COMPLEX BETWEEN PHOTOEXCITED RHODOPSIN AND TRANSDUCIN - RECIPROCAL INTERACTION BETWEEN THE RETINAL SITE IN RHODOPSIN AND THE NUCLEOTIDE SITE IN TRANSDUCIN [J].
BORNANCIN, F ;
PFISTER, C ;
CHABRE, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 184 (03) :687-698
[7]   Emerging role of homo- and heterodimerization in G-protein-coupled receptor biosynthesis and maturation [J].
Bulenger, S ;
Marullo, S ;
Bouvier, M .
TRENDS IN PHARMACOLOGICAL SCIENCES, 2005, 26 (03) :131-137
[8]   Biophysics - Is rhodopsin dimeric in native rods? [J].
Chabre, M ;
Cone, R ;
Saibil, H .
NATURE, 2003, 426 (6962) :30-31
[9]   Interaction of amphipols with sarcoplasmic reticulum Ca2+-ATPase [J].
Champeil, P ;
Menguy, T ;
Tribet, C ;
Popot, JL ;
le Maire, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (25) :18623-18637
[10]   Activation of G-protein Gα subunits by receptors through Gα-Gβ and Gα-Gγ interactions [J].
Cherfils, J ;
Chabre, M .
TRENDS IN BIOCHEMICAL SCIENCES, 2003, 28 (01) :13-17