Study on Apoptosis of Prostate Cancer Cells Induced by IκBα Overexpression Synergistic with Poly (Lactic-Co-Glycolic Acid)-Curcumin Nanoparticles

Objective. To investigate the synergistic effects of I kappa B alpha overexpression and poly (lactic-co-glycolic acid)-curcumin nanoparticles (PLGA-Cur-NPs) on prostate cancer (PC) and reveal the underlying mechanisms of cooperative sensitization induced by curcumin. Methods. Proliferation and apoptosis rate in I kappa B alpha overexpressed and PLGA-Cur-NPs-treated PC cells were detected by MTTand flow cytometry assay. The expression levels of I kappa B alpha, apoptosis-related, and signaling proteins were measured by western blotting assay. Results. PC cell proliferation was significantly inhibited by the overexpression of I kappa B alpha. The apoptosis rate of PC cells was significantly increased at a high concentration of curcumin exposure, while the activation of NF-kappa B pathway was obviously inhibited. In addition, PLGA-Cur-NPs treatment synergistic with I kappa B alpha overexpression enhanced the apoptosis of PC cells by suppressing the NF-kappa B pathway activation. Conclusion. I kappa B alpha overexpression synergistic with PLGA-Cur-NPs could obviously inhibit proliferation, induce apoptosis, and suppress the activation of NF-kappa B pathway in PC cells. These findings may provide an experimental basis to establish the tumor gene therapy combined with traditional Chinese medicine treatment, thus promoting the clinical application of both tumor gene therapy and anti-tumor Chinese medicine.