A Defined, Feeder-Free, Serum-Free System to Generate In Vitro Hematopoietic Progenitors and Differentiated Blood Cells from hESCs and hiPSCs

被引:49
作者
Salvagiotto, Giorgia [1 ]
Burton, Sarah [1 ]
Daigh, Christine A. [1 ]
Rajesh, Deepika [1 ]
Slukvin, Igor I. [2 ]
Seay, Nicholas J. [1 ]
机构
[1] Cellular Dynam Int Inc, Dept Res & Dev, Madison, WI USA
[2] Univ Wisconsin, Dept Pathol & Lab Med, Madison, WI USA
来源
PLOS ONE | 2011年 / 6卷 / 03期
关键词
EMBRYONIC STEM-CELLS; ENDOTHELIAL GROWTH-FACTOR; CD34(+) CELLS; EFFICIENT DIFFERENTIATION; VASCULAR DEVELOPMENT; PLATELET PRODUCTION; STROMAL CELLS; MOUSE; EXPANSION; MESODERM;
D O I
10.1371/journal.pone.0017829
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Human ESC and iPSC are an attractive source of cells of high quantity and purity to be used to elucidate early human development processes, for drug discovery, and in clinical cell therapy applications. To efficiently differentiate pluripotent cells into a pure population of hematopoietic progenitors we have developed a new 2-dimentional, defined and highly efficient protocol that avoids the use of feeder cells, serum or embryoid body formation. Here we showed that a single matrix protein in combination with growth factors and a hypoxic environment is sufficient to generate from pluripotent cells hematopoietic progenitors capable of differentiating further in mature cell types of different lineages of the blood system. We tested the differentiation method using hESCs and 9 iPSC lines generated from different tissues. These data indicate the robustness of the protocol providing a valuable tool for the generation of clinical-grade hematopoietic cells from pluripotent cells.
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页数:9
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