Vitrification of Mouse Embryo-Derived ICM Cells: A Tool for Preserving Embryonic Stem Cell Potential?

被引：0
作者：
Desai, N. [1 ]
Xu, J. [1 ]
Tsulaia, T. [1 ]
Szeptycki, J. [1 ]
Austin, C. [1 ]
Goldfarb, J. [1 ]
Falcone, T. [1 ]
机构：
[1] Cleveland Clin, Fertil Ctr, Dept OB GYN, Beachwood, OH USA
来源：
1ST INTERNATIONAL CONGRESS ON CONTROVERSIES IN CRYOPRESERVATION OF STEM CELLS, REPRODUCTIVE CELLS, TISSUE AND ORGANS (CRYO) | 2010年
关键词：
DERIVATION; CRYOPRESERVATION; LINES;
D O I：
暂无
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Embryonic stem (ES) cell lines are established from the inner cell mass (ICM) of the pre-implantation blastocyst. Vitrification technology presents new opportunities for preservation of embryo derived stem cells without first establishing a viable ESC cell line. This may be especially valuable for human embryonic stem cell banking, eliminating the ethical complications of storing viable human embryos for future stem cell derivation. This study tests the feasibility of cryopreserving ICM cells using vitrification. The current work suggests that vitrification using either the cryoloop or HSV straws was an effective tool for the preservation of ICMs. Vitrified-warmed ICMs had high survival rates and upon plating were able to from embryonic stem cell (ESC)-like colonies. Furthermore, ICM derived ESCs remained undifferentiated for several passages and demonstrated expression of typical stem cell markers SSEA1, Sox2 Oct 4 and Alkaline Phosphatase (AP). This is the first report on successful ICM vitrification and the subsequent derivation of ESC colonies.
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页码：41 / 45
页数：5
相关论文
共 14 条
[1]   Optimal Timing of Inner Cell Mass Isolation Increases the Efficiency of Human Embryonic Stem Cell Derivation and Allows Generation of Sibling Cell Lines [J].
Chen, Alice E. ;
Egli, Dieter ;
Niakan, Kathy ;
Deng, Jie ;
Akutsu, Hidenori ;
Yamaki, Mariko ;
Cowan, Chad ;
Fitz-Gerald, Claire ;
Zhang, Kun ;
Melton, Douglas A. ;
Eggan, Kevin .
CELL STEM CELL, 2009, 4 (02) :103-106
[2]   Cryoloop vitrification of human day 3 cleavage-stage embryos: post-vitrification development, pregnancy outcomes and live births [J].
Desai, Nina ;
Blackmon, Heather ;
Szeptycki, Julia ;
Goldfarb, James .
REPRODUCTIVE BIOMEDICINE ONLINE, 2007, 14 (02) :208-213
[3]   Methods for derivation of human embryonic stem cells [J].
Kim, HS ;
Oh, SK ;
Park, YB ;
Ahn, HJ ;
Sung, KC ;
Kang, MJ ;
Lee, LA ;
Suh, CS ;
Kim, SH ;
Kim, DW ;
Moon, SY .
STEM CELLS, 2005, 23 (09) :1228-1233
[4]   Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination [J].
Kuwayama, M ;
Vajta, G ;
Ieda, S ;
Kato, O .
REPRODUCTIVE BIOMEDICINE ONLINE, 2005, 11 (05) :608-614
[5]   Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique [J].
Lane, M ;
Schoolcraft, WB ;
Gardner, DK .
FERTILITY AND STERILITY, 1999, 72 (06) :1073-1078
[6]   Effect of carrier system on the yield of human oocytes and embryos as assessed by survival and developmental potential after vitrification [J].
Liebermann, J ;
Tucker, MJ .
REPRODUCTION, 2002, 124 (04) :483-489
[7]   ISOLATION OF A PLURIPOTENT CELL-LINE FROM EARLY MOUSE EMBRYOS CULTURED IN MEDIUM CONDITIONED BY TERATOCARCINOMA STEM-CELLS [J].
MARTIN, GR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (12) :7634-7638
[8]   Vitrification of human blastocysts using cryoloops: clinical outcome of 223 cycles [J].
Mukaida, T ;
Nakumura, S ;
Tomiyama, T ;
Wada, S ;
Oka, C ;
Kasai, M ;
Takahashi, K .
HUMAN REPRODUCTION, 2003, 18 (02) :384-391
[9]   Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification method [J].
Reubinoff, BE ;
Pera, MF ;
Vajta, G ;
Trounson, AO .
HUMAN REPRODUCTION, 2001, 16 (10) :2187-2194
[10]   An efficient and safe Xeno-Free Cryopreservation method for the storage of human embryonic stem cells [J].
Richards, M ;
Fong, CY ;
Tan, S ;
Chan, WK ;
Bongso, A .
STEM CELLS, 2004, 22 (05) :779-789
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