Improved Agrobacterium-mediated transformation of Vinca minor via combination of dynamic transfection and vacuum infiltration

An improved Agrobacterium-mediated transformation method was developed by combining dynamic transfection with vacuum infiltration and challenged for Vinca minor hairy roots induction. The transfection was conducted in a simple device including a funnel-shaped bottle, a vacuum pump and a liquid pump. During the process of transformation, the bacterial solution continuously circulated in the funnel-shaped bottle and the combination of dynamic transfection and vacuum infiltration was achieved by using the liquid pump and vacuum pump simultaneously. The results indicated that the transformation efficiency was affected by filter size, solution circulation velocity as well as vacuum level and the maximum transformation rate of 82.5% was obtained under the appropriate conditions which was higher than that 25.3% in control.