Dynamics of association of origins of DNA replication with the nuclear matrix during the cell cycle

被引：33

作者：

Djeliova, V ^{[1
]}

Russev, G ^{[1
]}

Anachkova, B ^{[1
]}

机构：

[1] Bulgarian Acad Sci, Inst Mol Biol, BU-1113 Sofia, Bulgaria

来源：

NUCLEIC ACIDS RESEARCH | 2001年 / 29卷 / 15期

关键词：

D O I：

10.1093/nar/29.15.3181

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA of replication foci attached to the nuclear matrix was isolated from Chinese hamster ovary cells and human HeLa cells synchronized at different stages of the G(1) and S phases of the cell cycle. The abundance of sequences from dihydrofolate reductase ori-beta and the beta -globin replicator was determined in matrix-attached DNA. The results show that matrix-attached DNA isolated from cells in late G(1) phase was enriched in origin sequences in comparison with matrix-attached DNA from early G(1) phase cells. The concentration of the early firing ori-beta in DNA attached to the matrix decreased in early S phase, while the late firing beta -globin origin remained attached until late S phase. We conclude that replication origins associate with the nuclear matrix in late G(1) phase and dissociate after initiation of DNA replication in S phase.

引用

页码：3181 / 3187

页数：7

共 47 条

[1] Genetic dissection of a mammalian replicator in the human β-globin locus [J].