Effect of centrally administered apelin-13 on gastric emptying and gastrointestinal transit in mice

被引：42
作者：
Lv, Shuang-Yu [1 ]
Yang, Yan-Jie [1 ]
Qin, Yao-Jun [1 ]
Xiong, Wei [1 ]
Chen, Qiang [1 ]
机构：
[1] Lanzhou Univ, Sch Life Sci, Inst Biochem & Mol Biol, Lanzhou 730000, Peoples R China
来源：
PEPTIDES | 2011年 / 32卷 / 05期
关键词：
Apelin-13; Gastric emptying; Gastrointestinal transit; Apelin-13(F13A); Opioid receptor; FOOD-INTAKE; PEPTIDE APELIN; MESSENGER-RNA; TISSUE DISTRIBUTION; OPIOID RECEPTOR; IN-VITRO; LIGAND; APJ; NEUROPEPTIDE; EXPRESSION;
D O I：
10.1016/j.peptides.2011.01.023
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Apelin, as the endogenous ligand for the APJ, regulates many biological functions, including blood pressure, neuroendocrine, drinking behavior, food intake and colonic motility. The present study was designed to investigate the effect of central apelin-13 on gastric emptying and gastrointestinal transit in mice. Intracerebroventricular (icy.) injection of apelin-13 (3 and 10 mu g/mouse) decreased gastric emptying rate by 10.9% and 17.1%. This effect was significantly antagonized by the APJ receptor antagonist apelin-13(F13A) and the opioid receptor antagonist naloxone, respectively. However, intraperitoneal (i.p.) injection of apelin-13 (10-100 mu g/mouse) did not affect gastric emptying. Apelin-13 (0.3, 1 and 3 mu g/mouse, i.c.v.) inhibited gastrointestinal transit by 16.8%, 23.4% and 19.2%. Apelin-13(F13A) and naloxone could also reverse this antitransit effect induced by apelin-13. Taken together, these results suggest that i.c.v. injected apelin-13 inhibits gastric emptying and gastrointestinal transit and it seems that APJ receptor and opioid receptor might be involved in these processes. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：978 / 982
页数：5
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