Discrimination and ultrasensitive detection of β2-agonists using copper nanoclusters as a fluorescent probe

被引：13
作者：
Chen, Miaomiao [1 ,2 ]
Li, Weiming [1 ,2 ]
Xiong, Huayu [1 ,2 ]
Wen, Wei [1 ,2 ]
Zhang, Xiuhua [1 ,2 ]
Wang, Shengfu [1 ,2 ]
机构：
[1] Hubei Univ, Hubei Collaborat Innovat Ctr Adv Organ Chem Mat, Key Lab Synth & Applicat Organ Funct Mol, Minist Educ, Wuhan 430062, Hubei, Peoples R China
[2] Hubei Univ, Coll Chem & Chem Engn, Wuhan 430062, Hubei, Peoples R China
来源：
MICROCHIMICA ACTA | 2017年 / 184卷 / 09期
基金：
中国国家自然科学基金;
关键词：
Fluorometric assay; Fluorescence quenching; Colorimetric assay; Quenching mechanism; GOLD NANOCLUSTERS; CARCASS CHARACTERISTICS; SILVER NANOCLUSTERS; SENSITIVE DETECTION; ENERGY-TRANSFER; BETA-AGONISTS; CARBON DOTS; LABEL-FREE; RECOGNITION; SENSOR;
D O I：
10.1007/s00604-017-2357-2
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The authors describe a rapid method for the determination of 11 beta(2)-agonists by exploiting the color changes and fluorescence intensity changes of copper nanoclusters coated with bovine serum albumin (BSA-CuNCs). On addition to a solution of the BSA-CuNCs, some beta(2)-agonists (metaproterenol, terbutaline, dopamine hydrochloride, isoproterenol, fenoterol) cause color transitions to occur, while the others do not cause an effect. In addition, beta(2)-agonists such as isoxsuprine, ritodrine, ractopamine, metaproterenol, terbutaline, dopamine, isoproterenol and fenoterol quench the fluorescence of the CuNCs (best measured at excitation/emission wavelengths of 325/410 nm), while the others do not quench. By combining the effects on color and fluorescence, one can differentiate the various types of agonists. The possible quenching mechanism is investigated. The strong decrease in fluorescence intensity upon addition of certain beta(2)-agonists can also be used quantify the concentration of the beta(2)-agonists.
引用
收藏
页码：3317 / 3324
页数：8
相关论文
共 25 条
[1]   Highly Sensitive Emission Sensor Based on Surface Plasmon Enhanced Energy Transfer between Gold Nanoclusters and Silver Nanoparticles [J].
Chen, Chih-Wei ;
Wang, Chun-Hsiung ;
Wei, Chih-Ming ;
Hsieh, Chun-Yi ;
Chen, Yung-Ting ;
Chen, Yang-Fang ;
Lai, Chih-Wei ;
Liu, Chien-Liang ;
Hsieh, Cheng-Chih ;
Chou, Pi-Tai .
JOURNAL OF PHYSICAL CHEMISTRY C, 2010, 114 (02) :799-802
[2]   Dual Recognition Strategy for Specific and Sensitive Detection of Bacteria Using Aptamer-Coated Magnetic Beads and Antibiotic-Capped Gold Nanoclusters [J].
Cheng, Dan ;
Yu, Mengqun ;
Fu, Fei ;
Han, Weiye ;
Li, Gan ;
Xie, Jianping ;
Song, Yang ;
Swihart, Mark T. ;
Song, Erqun .
ANALYTICAL CHEMISTRY, 2016, 88 (01) :820-825
[3]   Luminescent Silver Nanoclusters Acting as a Label-Free Photoswitch in Metal Ion Sensing [J].
Ghosh, Subhadip ;
Anand, Uttam ;
Mukherjee, Saptarshi .
ANALYTICAL CHEMISTRY, 2014, 86 (06) :3188-3194
[4]   Effect of ractopamine-HCl supplementation for 28 days on carcass characteristics, muscle fiber morphometrics, and whole muscle yields of six distinct muscles of the loin and round [J].
Gonzalez, J. M. ;
Johnson, S. E. ;
Stelzleni, A. M. ;
Thrift, T. A. ;
Savell, J. D. ;
Warnock, T. M. ;
Johnson, D. D. .
MEAT SCIENCE, 2010, 85 (03) :379-384
[5]   Copper Quantum Clusters in Protein Matrix: Potential Sensor of Pb2+ Ion [J].
Goswami, Nirmal ;
Giri, Anupam ;
Bootharaju, M. S. ;
Xavier, Paulrajpillai Lourdu ;
Pradeep, Thalappil ;
Pal, Samir Kumar .
ANALYTICAL CHEMISTRY, 2011, 83 (24) :9676-9680
[6]   Probing the interaction between two single molecules: Fluorescence resonance energy transfer between a single donor and a single acceptor [J].
Ha, T ;
Enderle, T ;
Ogletree, DF ;
Chemla, DS ;
Selvin, PR ;
Weiss, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (13) :6264-6268
[7]   Direct Detection of β-Agonists by Use of Gold Nanoparticle-Based Colorimetric Assays [J].
He, Pingli ;
Shen, Li ;
Liu, Rongyuan ;
Luo, Zhiping ;
Li, Zhen .
ANALYTICAL CHEMISTRY, 2011, 83 (18) :6988-6995
[8]   Highly selective fluorescent sensors for Hg2+ based on bovine serum albumin-capped gold nanoclusters [J].
Hu, Dehong ;
Sheng, Zonghai ;
Gong, Ping ;
Zhang, Pengfei ;
Cai, Lintao .
ANALYST, 2010, 135 (06) :1411-1416
[9]   Electrochemical immunosensor based on polyaniline/poly (acrylic acid) and Au-hybrid graphene nanocomposite for sensitivity enhanced detection of salbutamol [J].
Huang, Jiadong ;
Lin, Qing ;
Zhang, Xiuming ;
He, Xiaorui ;
Xing, Xianrong ;
Lian, Wenjing ;
Zuo, Mingming ;
Zhang, Qingqing .
FOOD RESEARCH INTERNATIONAL, 2011, 44 (01) :92-97
[10]   A carbon dots based fluorescent probe for selective and sensitive detection of hemoglobin [J].
Huang, Shan ;
Wang, Lumin ;
Huang, Chusheng ;
Xie, Jiangning ;
Su, Wei ;
Sheng, Jiarong ;
Xiao, Qi .
SENSORS AND ACTUATORS B-CHEMICAL, 2015, 221 :1215-1222
123