Baker's Yeast Transformation Studies by Atomic Force Microscopy

被引：12

作者：

Suchodolskis, Arturas ^{[1
]}

Stirke, Arunas ^{[1
]}

Timonina, Ana ^{[1
]}

Ramanaviciene, Almira ^{[1
,2
]}

Ramanavicius, Arunas ^{[1
,3
]}

机构：

[1] Vilnius Univ, Fac Chem, Ctr Nanotechnol & Mat Sci NanoTechnas, LT-03225 Vilnius, Lithuania

[2] Ctr Innovat Med, Div Immunotechnol, LT-01102 Vilnius, Lithuania

[3] State Res Inst Ctr Phys & Technol Sci, Inst Chem, LT-01108 Vilnius, Lithuania

来源：

ADVANCED SCIENCE LETTERS | 2011年 / 4卷 / 01期

关键词：

AFM; Baker's Yeast; Electroporation; Transformation; Living Cell; QUANTUM DOTS; INITIATED POLYMERIZATION; SACCHAROMYCES-CEREVISIAE; CELLULAR UPTAKE; BIOFUEL CELLS; NANOPARTICLES; BIOCOMPATIBILITY; NANOSECOND; ETHANOL; ENZYME;

D O I：

10.1166/asl.2011.1189

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Baker's yeast cells (Saccharomyces cerevisiae) before and after milli-second pulse electroporation procedure were imaged by Atomic Force Microscopy. Morphological changes of outer cell wall were revealed. We clearly observed induced irregularities on walls, which we attributed to holes induced by transformation following electroporation while cells surface prior to electroporation was smooth.

引用

页码：171 / 173

页数：3

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