0610009K11Rik, a testis-specific and germ cell nuclear receptor-interacting protein

被引：5

作者：

Zhang, Heng ^{[1
]}

Denhard, Leslie A. ^{[1
]}

Zhou, Huaxin ^{[1
]}

Liu, Lan-Hsin ^{[1
]}

Lan, Zi-Jian ^{[1
]}

机构：

[1] Univ Louisville, Hlth Sci Ctr, Birth Defects Ctr, Louisville, KY 40202 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 366卷 / 04期

关键词：

spermatocyte; spermatids; GCNF; nuclear receptor; testis; mouse; in silico; protein-protein interaction;

D O I：

10.1016/j.bbrc.2007.12.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using an in silico approach, a putative nuclear receptor-interacting protein 0610009K11Rik was identified in mouse testis. We named this gene testis-specific nuclear receptor-interacting protein-1 (Tnrip-1). Tnrip-1 was predominantly expressed in the testis of adult mouse tissues. Expression of Tnrip-1 in the testis was regulated during postnatal development, with robust expression in 14-day-old or older testes. In situ hybridization analyses showed that Tnrip-1 is highly expressed in pachytene spermatocytes and spermatids. Consistent with its mRNA expression, Tnrip-1 protein was detected in adult mouse testes. Immunohistochemical studies showed that Tnrip-1 is a nuclear protein and mainly expressed in pachytene spermatocytes and round spermatids. Moreover, co-immunoprecipitation analyses showed that endogenous Tnrip-1 protein can interact with germ cell nuclear receptor (GCNF) in adult mouse testes. Our results suggest that Tnrip-1 is a testis-specific and GCNF-interacting protein which may be involved in the modulation of GCNF-mediated gene transcription in spermatogenic cells within the testis. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：898 / 904

页数：7