High-efficiency cloning of Arabidopsis full-length cDNA by biotinylated CAP trapper

被引：184

作者：

Seki, M

Carninci, P

Nishiyama, Y

Hayashizaki, Y

Shinozaki, K

机构：

[1] RIKEN, Inst Phys & Chem Res, Plant Mol Biol Lab, Tsukuba Life Sci Ctr, Tsukuba, Ibaraki 3050074, Japan

[2] RIKEN, Inst Phys & Chem Res, Genome Sci Lab, Tsukuba Life Sci Ctr, Tsukuba, Ibaraki 3050074, Japan

[3] JST, CREST, Kawaguchi, Saitama 332, Japan

来源：

PLANT JOURNAL | 1998年 / 15卷 / 05期

关键词：

D O I：

10.1046/j.1365-313x.1998.00237.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Full-length cDNAs are essential for functional analysis of plant genes. We constructed high-content, full-length cDNA libraries from Arabidopsis thaliana plants based on chemical introduction of a biotin group into the diol residue of the CAP structure of eukaryotic mRNA, followed by RNase I treatment, to select full-length cDNA. More than 90% of the total clones obtained were of full length; recombinant clones were obtained with high efficiency (2.2 x 10(6)/9 mu g starting mRNA). Sequence analysis of 111 randomly picked clones indicated that 32 isolated cDNA groups were derived from novel genes in the A. thaliana genome.

引用

页码：707 / 720

页数：14

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