Purification and properties of an active site mutant, H48Q, of human non-pancreatic secreted phospholipase A2

被引：7
作者：
Edwards, SH [1 ]
Baker, SF [1 ]
Wilton, DC [1 ]
机构：
[1] Univ Southampton, Sch Biol Sci, Div Biochem & Mol Biol, Southampton SO16 7PX, Hants, England
来源：
BIOCHEMICAL SOCIETY TRANSACTIONS | 1998年 / 26卷 / 03期
关键词：
D O I：
10.1042/bst026s239
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
[No abstract available]
引用
收藏
页码：S239 / S239
页数：1
相关论文
共 6 条
  • [1] Active site of bee venom phospholipase A(2): The role of histidine-34, aspartate-64 and tyrosine-87
    Annand, RR
    Kontoyianni, M
    Penzotti, JE
    Dudler, T
    Lybrand, TP
    Gelb, MH
    [J]. BIOCHEMISTRY, 1996, 35 (14) : 4591 - 4601
  • [2] PHOSPHOLIPASE-A2 ENGINEERING .10. THE ASPARTATE...HISTIDINE CATALYTIC DIAD ALSO PLAYS AN IMPORTANT STRUCTURAL ROLE
    LI, YS
    TSAI, MD
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (19) : 8523 - 8526
  • [3] Human non-pancreatic (group II) secreted phospholipase A(2) expressed from a synthetic gene in Escherichia coli: Characterisation of N-terminal mutants
    Othman, R
    Baker, S
    Li, Y
    Worrall, AF
    Wilton, DC
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1996, 1303 (02): : 92 - 102
  • [4] High specificity of human secretory class II phospholipase A(2) for phosphatidic acid
    Snitko, Y
    Yoon, ET
    Cho, WH
    [J]. BIOCHEMICAL JOURNAL, 1997, 321 : 737 - 741
  • [5] VADAS P, 1993, J LIPID MEDIATOR, V8, P1
  • [6] STRUCTURE OF RECOMBINANT HUMAN RHEUMATOID ARTHRITIC SYNOVIAL-FLUID PHOSPHOLIPASE-A2 AT 2.2 A RESOLUTION
    WERY, JP
    SCHEVITZ, RW
    CLAWSON, DK
    BOBBITT, JL
    DOW, ER
    GAMBOA, G
    GOODSON, T
    HERMANN, RB
    KRAMER, RM
    MCCLURE, DB
    MIHELICH, ED
    PUTNAM, JE
    SHARP, JD
    STARK, DH
    TEATER, C
    WARRICK, MW
    JONES, ND
    [J]. NATURE, 1991, 352 (6330) : 79 - 82
1