Fast and sensitive protein alignment using DIAMOND

被引：8812

作者：

Buchfink, Benjamin ^{[1
,2
]}

Xie, Chao ^{[3
,4
]}

Huson, Daniel H. ^{[1
,2
,3
]}

机构：

[1] Univ Tubingen, Dept Comp Sci, Tubingen, Germany

[2] Univ Tubingen, Ctr Bioinformat, Tubingen, Germany

[3] Nanyang Technol Univ, Sch Biol Sci, Singapore Ctr Environm Life Sci Engn, Singapore 639798, Singapore

[4] Natl Univ Singapore, Inst Life Sci, Singapore 117548, Singapore

来源：

NATURE METHODS | 2015年 / 12卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

SEARCH;

D O I：

10.1038/nmeth.3176

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The alignment of sequencing reads against a protein reference database is a major computational bottleneck in metagenomics and data-intensive evolutionary projects. Although recent tools offer improved performance over the gold standard BLASTX, they exhibit only a modest speedup or low sensitivity. We introduce DIAMOND, an open-source algorithm based on double indexing that is 20,000 times faster than BLASTX on short reads and has a similar degree of sensitivity.

引用

页码：59 / 60

页数：2

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