Synthetic Vehicles for Encapsulation and Delivery of CRISPR/Cas9 Gene Editing Machinery

Clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated 9 (Cas-9) technology holds tremendous potential as a gene editing tool. Different strategies have been adopted for in vitro and in vivo delivery of CRISPR/Cas9, including both viral and non-viral. The possibility of tailoring properties of nanosized systems makes the molecular design of self-assembled non-viral delivery systems based on organic (lipids and polymers) and hybrid (zeolitic imidazolate frameworks, ZIF and gold nanoparticles) materials of a great interest in CRISPR/Cas9 delivery. This review highlights the progress and challenges of organic and hybrid CRISPR/Cas9 delivery vehicles.