Investigation of factors affecting xylanase activity from Trichoderma harzianum 1073 D3

被引：21
|
作者：
Isil, S [1 ]
Nilufer, A [1 ]
机构：
[1] Hacettepe Univ, Dept Biol Biotechnol, TR-06532 Ankara, Turkey
来源：
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2005年 / 48卷 / 02期
关键词：
Trichoderma harzianum; xylan; xylanase; lignocellulosic material; enzyme stability; metal ions;
D O I：
10.1590/S1516-89132005000200004
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In this study, some physiological conditions affecting the activity of xylanase enzyme produced from Trichoderma harzianum 1073 D3 were determined. In addition, stabilization of pH and temperature in liquid and semi-solid state cultivation media were investigated. It was concluded that for maximum xylanase activity incubation at 60 degrees C in an enzyme incubation medium with pH 5 that contained 1% xylan was appropriate. The stability studies showed that the enzyme was relatively stable in the pH range 3-7 and retained more than 50% of its original activity after four months.
引用
收藏
页码：187 / 193
页数：7
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