Cardioprotective properties of Crataegus oxycantha extract against ischemia-reperfusion injury

被引:40
作者
Swaminathan, J. K. [1 ,2 ]
Khan, M. [1 ]
Mohan, I. K. [1 ]
Selvendiran, K. [1 ]
Devaraj, S. Niranjali [2 ]
Rivera, B. K. [1 ]
Kuppusamy, P. [1 ]
机构
[1] Ohio State Univ, Dept Internal Med, Ctr Biomed EPR Spect & Imaging, Davis Heart & Lung Res Inst, Columbus, OH 43210 USA
[2] Univ Madras, Dept Biochem, Madras 600025, Tamil Nadu, India
关键词
Oxidative stress; Infarction; Ischemia-reperfusion injury; Rat heart; Crataegus oxycantha; Apoptosis; INDUCED MYOCARDIAL-INFARCTION; OXIDATIVE STRESS; GENE-EXPRESSION; RAT MYOCARDIUM; CELL-DEATH; HAWTHORN EXTRACT; HEART-FAILURE; APOPTOSIS; ACTIVATION; CARDIOMYOCYTES;
D O I
10.1016/j.phymed.2010.01.009
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The aim of the study was to investigate the cardioprotective effect and mechanism of Crataegus oxycantha (COC) extract, a well-known natural antioxidant-based cardiotonic, against ischemia/reperfusion (I/R) injury. Electron paramagnetic resonance studies showed that COC extract was capable of scavenging superoxide, hydroxyl, and peroxyl radicals, in vitro. The cardioprotective efficacy of the extract was studied in a crystalloid perfused heart model of I/R injury. Hearts were subjected to 30 min of global ischemia followed by 45 min of reperfusion. During reperfusion, COC extract was infused at a dose rate of 1 mg/ml/min for 10 min. Hearts treated with COC extract showed a significant recovery in cardiac contractile function, reduction in infarct size, and decrease in creatine kinase and lactate dehydrogenase activities. The expressions of xanthine oxidase and NADPH oxidase were significantly reduced in the treated group. A significant upregulation of the anti-apoptotic proteins Bcl-2 and Hsp70 with simultaneous downregulation of the pro-apoptotic proteins cytochrome c and cleaved caspase-3 was observed. The molecular signaling cascade including phospho-Akt (ser-473) and HIF-1 alpha that lead to the activation or suppression of apoptotic pathway also showed a significant protective role in the treatment group. No significant change in phospho-p38 levels was observed. The results suggested that the COC extract may reduce the oxidative stress in the reperfused myocardium, and play a significant role in the inhibition of apoptotic pathways leading to cardioprotection. (C) 2010 Elsevier GmbH. All rights reserved.
引用
收藏
页码:744 / 752
页数:9
相关论文
共 50 条
  • [41] Cardioprotective Utility of Urocortin in Myocardial Ischemia-Reperfusion Injury: Where do We Stand?
    Basman, Craig
    Agrawal, Pratik
    Knight, Richard
    Saravolatz, Louis
    Mcree, Chad
    Chen-Scarabelli, Carol
    Narula, Jagat
    Scarabelli, Tiziano
    CURRENT MOLECULAR PHARMACOLOGY, 2018, 11 (01) : 32 - 38
  • [42] Effect of carvedilol against myocardial injury due to ischemia-reperfusion of the brain in rats
    Harima, Meilei
    Arumugam, Somasundaram
    Wen, Juan
    Pitchaimani, Vigneshwaran
    Karuppagounder, Vengadeshprabhu
    Afrin, Mst. Rejina
    Sreedhar, Remya
    Miyashita, Shizuka
    Nomoto, Mayumi
    Ueno, Kazuyuki
    Nakamura, Masahiko
    Watanabe, Kenichi
    EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2015, 98 (03) : 558 - 562
  • [43] Prostaglandins mediate the cardioprotective effects of atorvastatin against ischemia-reperfusion injury
    Birnbaum, Y
    Ye, YM
    Rosanio, S
    Tavackoli, S
    Hu, ZY
    Schwarz, ER
    Uretsky, BF
    CARDIOVASCULAR RESEARCH, 2005, 65 (02) : 345 - 355
  • [44] Ischemic postconditioning provides cardioprotective and antiapoptotic effects against ischemia-reperfusion injury through iNOS inhibition in hyperthyroid rats
    Zaman, Jalal
    Jeddi, Sajad
    Daneshpour, Maryam Sadat
    Zarkesh, Maryam
    Daneshian, Zahra
    Ghasemi, Asghar
    GENE, 2015, 570 (02) : 185 - 190
  • [45] Loss of clusterin expression worsens renal ischemia-reperfusion injury
    Zhou, Wenjun
    Guan, Qiunong
    Kwan, Chris C. H.
    Chen, Huifang
    Gleave, Martin E.
    Nguan, Christopher Y. C.
    Du, Caigan
    AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2010, 298 (03) : F568 - F578
  • [46] Protective effect of morin on myocardial ischemia-reperfusion injury in rats
    Liu, Shuang
    Wu, Nan
    Miao, Jiaxin
    Huang, Zijun
    Li, Xuying
    Jia, Pengyu
    Guo, Yuxuan
    Jia, Dalin
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2018, 42 (03) : 1379 - 1390
  • [47] Protective Effects and Mechanisms of Flavonoids in Renal Ischemia-Reperfusion Injury
    Peng, Peng
    Zou, Junrong
    Zhong, Bin
    Zhang, Guoxi
    Zou, Xiaofeng
    Xie, Tianpeng
    PHARMACOLOGY, 2023, 108 (01) : 27 - 36
  • [48] Spatial Multiplexed Protein Profiling of Cardiac Ischemia-Reperfusion Injury
    Yao, Luyan
    He, Funan
    Zhao, Quanyi
    Li, Dandan
    Fu, Shufang
    Zhang, Mingzhi
    Zhang, Xingzhong
    Zhou, Bingying
    Wang, Li
    CIRCULATION RESEARCH, 2023, 133 (01) : 86 - 103
  • [49] Aortic ischemia-reperfusion injury and potency of fluoxetine
    Altan, Mehmet
    Yaman, Muhittin Onur
    Kervancioglu, Gulnaz
    Kilic, Aysu
    Demirci, Elif Kervancioglu
    polat, Sila Hidayet Bozdogan
    Karadeniz, Zeliha
    Guner, Ibrahim
    Yelmen, Nermin
    Sahin, Gulderen
    IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 2023, 26 (03) : 301 - 307
  • [50] Protective Effect of Hydrogen Sulfide on Cerebral Ischemia-Reperfusion Injury
    Deng, Gang
    Muqadas, Masood
    Adlat, Salah
    Zheng, Haiyun
    Li, Ge
    Zhu, Ping
    Nasser, M., I
    CELLULAR AND MOLECULAR NEUROBIOLOGY, 2023, 43 (01) : 15 - 25