Over-Expression of the Cell-Cycle Gene LaCDKB1;2 Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during Larix kaempferi Somatic Embryogenesis

被引:14
作者
Kang, Yanhui [1 ]
Li, Wanfeng [1 ]
Zhang, Lifeng [1 ]
Qi, Liwang [1 ]
机构
[1] Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm,Natl Forestry & Gra, Beijing 100091, Peoples R China
基金
中国国家自然科学基金;
关键词
Larix kaempferi; LaCDKB1; 2; cell-cycle gene; somatic embryogenesis; X L-DECIDUA; ABSCISIC-ACID; PLANT-REGENERATION; ZYGOTIC EMBRYOS; MATURATION; CULTURES; ABA; OVEREXPRESSION; BIOSYNTHESIS; LAMIR166A;
D O I
10.3390/genes12091435
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Somatic embryogenesis is an effective tool for the production of forest tree seedlings with desirable characteristics; however, the low initiation frequency and productivity of high-quality mature somatic embryos are still limiting factors for Larix kaempferi (Japanese larch). Here, we analyzed the expression pattern of L. kaempferi cyclin-dependent kinase B 1;2 (LaCDKB1;2) during somatic embryogenesis in L. kaempferi and its relationship with the cell proliferation rate. We also analyzed the effect of LaCDKB1;2 over-expression on somatic embryo quality. The results revealed a positive correlation between LaCDKB1;2 expression and the cell proliferation rate during the proliferation stage. After LaCDKB1;2 over-expression, the proliferation rate of cultures increased, and the number of somatic embryos in transgenic cultures was 2.69 times that in non-transformed cultures. Notably, the number of normal cotyledonary embryos in transgenic cultures was 3 times that in non-transformed cultures, indicating that LaCDKB1;2 not only increases the proliferation of cultures and the number of somatic embryos but also improves the quality of somatic embryos. These results provide insight into the regulatory mechanisms of somatic embryogenesis as well as new Larix breeding material.
引用
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页数:14
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