Optical Control of Neuronal Activity

被引:88
作者
Szobota, Stephanie [1 ]
Isacoff, Ehud Y. [1 ,2 ,3 ]
机构
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Phys Biosci, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Mat Sci, Berkeley, CA 94720 USA
来源
ANNUAL REVIEW OF BIOPHYSICS, VOL 39 | 2010年 / 39卷
关键词
photostimulation; optogenetics; SPARK; LiGluR; channelrhodopsin; halorhodopsin; LIGHT-INDUCED ACTIVATION; REMOTE-CONTROL; ECTOPIC EXPRESSION; ION CHANNELS; MILLISECOND-TIMESCALE; OPTOGENETIC CONTROL; LASER STIMULATION; NEURAL CIRCUITRY; CAGED GLUTAMATE; K+ CHANNEL;
D O I
10.1146/annurev.biophys.093008.131400
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Advances in optics, genetics, and chemistry have enabled the investigation of brain function at all levels, from intracellular signals to single synapses, whole cells, circuits, and behavior. Until recent years, these research tools have been utilized in an observational capacity: imaging neural activity with fluorescent reporters, for example, or correlating aberrant neural activity with loss-of-function and gain-of-function pharmacological or genetic manipulations. However, optics, genetics, and chemistry have now combined to yield a new strategy: using light to drive and halt neuronal activity with molecular specificity and millisecond precision. Photostimulation of neurons is noninvasive, has unmatched spatial and temporal resolution, and can be targeted to specific classes of neurons. The optical methods developed to date encompass a broad array of strategies, including photorelease of caged neurotransmitters, engineered light-gated receptors and channels, and naturally light-sensitive ion channels and pumps. In this review, we describe photostimulation methods, their applications, and opportunities for further advancement.
引用
收藏
页码:329 / 348
页数:20
相关论文
共 98 条
[1]   Neural substrates of awakening probed with optogenetic control of hypocretin neurons [J].
Adamantidis, Antoine R. ;
Zhang, Feng ;
Aravanis, Alexander M. ;
Deisseroth, Karl ;
De Lecea, Luis .
NATURE, 2007, 450 (7168) :420-U9
[2]   Effect of Three Different Intensities of Infrared Laser Energy on the Levels of Amino Acid Neurotransmitters in the Cortex and Hippocampus of Rat Brain [J].
Ahmed, Nawal Abd El Hay ;
Radwan, Nasr Mahmoud ;
Ibrahim, Khayria Mansour ;
Khedr, Mona Emam ;
El Aziz, Mona A. ;
Khadrawy, Yasser Ashry .
PHOTOMEDICINE AND LASER SURGERY, 2008, 26 (05) :479-488
[3]   Temporally precise in vivo control of intracellular signalling [J].
Airan, Raag D. ;
Thompson, Kimberly R. ;
Fenno, Lief E. ;
Bernstein, Hannah ;
Deisseroth, Karl .
NATURE, 2009, 458 (7241) :1025-1029
[4]   Light-Induced Rescue of Breathing after Spinal Cord Injury [J].
Alilain, Warren J. ;
Li, Xiang ;
Horn, Kevin P. ;
Dhingra, Rishi ;
Dick, Thomas E. ;
Herlitze, Stefan ;
Silver, Jerry .
JOURNAL OF NEUROSCIENCE, 2008, 28 (46) :11862-11870
[5]   STIMULATION IN THE RAT OF A NERVE-FIBER BUNDLE BY A SHORT UV PULSE FROM AN EXCIMER-LASER [J].
ALLEGRE, G ;
AVRILLIER, S ;
ALBEFESSARD, D .
NEUROSCIENCE LETTERS, 1994, 180 (02) :261-264
[6]   In vivo light-induced activation of neural circuitry in transgenic mice expressing channelrhodopsin-2 [J].
Arenkiel, Benjamin R. ;
Peca, Joao ;
Davison, Ian G. ;
Feliciano, Catia ;
Deisseroth, Karl ;
Augustine, George J. ;
Ehlers, Michael D. ;
Feng, Guoping .
NEURON, 2007, 54 (02) :205-218
[7]   Optical control of zebrafish behavior with halorhodopsin [J].
Arrenberg, Aristides B. ;
Del Bene, Filippo ;
Baier, Herwig .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (42) :17968-17973
[8]   Spectral characteristics of the photocycle of channelrhodopsin-2 and its implication for channel function [J].
Bamann, Christian ;
Kirsch, Taryn ;
Nagel, Georg ;
Bamberg, Ernst .
JOURNAL OF MOLECULAR BIOLOGY, 2008, 375 (03) :686-694
[9]   Light-activated ion channels for remote control of neuronal firing [J].
Banghart, M ;
Borges, K ;
Isacoff, E ;
Trauner, D ;
Kramer, RH .
NATURE NEUROSCIENCE, 2004, 7 (12) :1381-1386
[10]   Photochromic Blockers of Voltage-Gated Potassium Channels [J].
Banghart, Matthew R. ;
Mourot, Alexandre ;
Fortin, Doris L. ;
Yao, Jennifer Z. ;
Kramer, Richard H. ;
Trauner, Dirk .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (48) :9097-9101