Defective Histone H3K27 Trimethylation Modification in Embryos Derived from Heated Mouse Sperm

被引：5
作者：
Chao, Shi-Bin [1 ,2 ]
Chen, Lei [2 ]
Li, Jian-Chun [1 ]
Ou, Xiang-Hong [2 ]
Huang, Xiao-Jin [1 ]
Wen, Shu [3 ]
Sun, Qing-Yuan [2 ]
Gao, Guo-Lan [1 ,4 ]
机构：
[1] NanChang Univ, Affiliated Hosp 1, Ctr Reprod Med, Nanchang, Peoples R China
[2] Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
[3] Baylor Coll Med, Dept Obstet & Gynecol, Houston, TX 77030 USA
[4] Aviat Gen Hosp China, Dept Obstet & Gynecol, Beijing, Peoples R China
来源：
MICROSCOPY AND MICROANALYSIS | 2012年 / 18卷 / 03期
关键词：
intracytoplasmic sperm injection (ICSI); epigenetic reprogramming; DNA methylation; histone modification; inner cell mass (ICM); DNA METHYLATION; INTRACYTOPLASMIC INJECTION; CHROMATIN-STRUCTURE; NORMAL MICE; MICROINJECTION; PLURIPOTENCY; ACTIVATION; EXPRESSION; INTEGRITY; NUCLEI;
D O I：
10.1017/S1431927612000396
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
The mouse sperm genome is resistant to in vitro heat treatment, and embryos derived from heated sperm can support full-term embryonic development, but the blastocyst rate and implantation rate are lower compared to embryos derived from fresh sperm. In the present study, the patterns of DNA methylation, histone H4K12 (ACH4K12) acetylation, H3K9 trimethylation (H3K9-TriM), and H3K27 trimethylation (H3K27-TriM) in preimplantation embryos derived from 658C-heated sperm were investigated. Although no evident changes in global DNA methyaltion, histone H4K12 (ACH4K12) acetylation, and H3K9 trimethylation (H3K9-TriM) were found, significantly lower levels of H3K27-TriM, which was thought to be one of the reasons for low efficiency of mouse cloning, were found in the inner cell mass of heated-sperm derived blastocysts. Thus, defective modification of H3K27-TriM might contribute to compromised development of embryos derived from heated sperm.
引用
收藏
页码：476 / 482
页数：7
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