The Methanol Extract of Azadirachta indica A. Juss Leaf Protects Mice Against Lethal Endotoxemia and Sepsis

被引：10

作者：

Kim, Woong-Hyun ^{[2
]}

Song, Hyun-Ok ^{[2
]}

Jin, Chun Mei ^{[2
]}

Hur, Jong Moon ^{[3
]}

Lee, Hwa Sung ^{[3
]}

Jin, Han Yong ^{[2
]}

Kim, Sung Yeon ^{[1
]}

Park, Hyun ^{[2
]}

机构：

[1] Wonkwang Univ, Coll Pharm, Inst Pharmaceut Res & Dev, Iksan 570749, South Korea

[2] Wonkwang Univ, Sch Med, Zoonosis Res Ctr, Dept Infect Biol, Iksan 570749, South Korea

[3] Globalherb Co Ltd, Inst Oriental Med Sci, Andong 760801, South Korea

来源：

BIOMOLECULES & THERAPEUTICS | 2012年 / 20卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Sepsis; Azadirachta indica A. Juss; Rutin; NO; TNF-alpha; LPS; TUMOR-NECROSIS-FACTOR; NITRIC-OXIDE; SHOCK; TRANSCRIPTION; ACTIVATION; FLAVONOIDS; RESPONSES; CYTOKINE;

D O I：

10.4062/biomolther.2012.20.1.096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, the inhibitory effect of neem leaf extract (NLE) on lipopolysaccaride (LPS)-induced nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) production was examined both in vitro and in vivo. In vitro study revealed that NLE treatment (100 mu g/ml) inhibits LPS (100 ng/ml)-induced NO production by 96% and TNF-alpha production by 32%. The reduction in NO production is probably conferred by the complete suppression of inducible nitric oxide synthase (iNOS) expression. Interestingly, in vivo NLE significantly improved the survival rate of mice in an experimental sepsis model. Administration of NLE (100 mg/kg) 24 h before LPS treatment (20 mg/kg) improved the survival rate of mice by 60%. The inhibition of plasma NO and TNF-alpha production by NLE is likely to account for the improved survival of mice. Our results suggest that NLE may present a promising avenue in the development of therapeutic agents for the treatment of inflammatory diseases.

引用

页码：96 / 103

页数：8

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