Characterization of a β-glucosidase from Sulfolobus solfataricus for isoflavone glycosides

被引：40
|
作者：
Kim, Bi-Na [1 ]
Yeom, Soo-Jin [1 ]
Kim, Yeong-Su [1 ]
Oh, Deok-Kun [1 ]
机构：
[1] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 01期
基金：
新加坡国家研究基金会;
关键词：
Biotransformation; Daidzein; Daidzin; Isoflavone hydrolysis; Sulfolobus solfataricus; BACILLUS-SUBTILIS NATTO; HYDROLYSIS; PURIFICATION; AGLYCONES; SOYMILK;
D O I：
10.1007/s10529-011-0739-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The specific activity of a recombinant beta-glucosidase from Sulfolobus solfataricus for isoflavones was: daidzin > glycitin > genistin > malonyl genistin > malonyl daidzin > malonyl glycitin. The hydrolytic activity of this enzyme for daidzin was highest at pH 5.5 and 90A degrees C with a half-life of 18 h, a K (m) of 0.5 mM, and a k (cat) of 2532 s(-1). The enzyme converted 1 mM daidzin to 1 mM daidzein after 1 h with a molar yield of 100% and a productivity of 1 mM h(-1). Among beta-glucosidases, that from S. solfataricus beta had the highest thermostability, k (cat), k (cat)/K (m), conversion yield, and productivity in the hydrolysis of daidzin.
引用
收藏
页码：125 / 129
页数：5
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