A visual and sensitive Hg2+ detection strategy based on split DNAzyme amplification and peroxidase-like activity of hemin-graphene composites

被引：22

作者：

Deng, Pengxi ^{[1
]}

Zheng, Shuang ^{[2
]}

Yun, Wen ^{[2
]}

Zhang, Weilu ^{[3
]}

Yang, Lizhu ^{[1
]}

机构：

[1] Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China

[2] Chongqing Technol & Business Univ, Coll Environm & Resources, Chongqing Key Lab Catalysis & New Environm Mat, Chongqing 400067, Peoples R China

[3] Wenzhou Univ, Coll Chem & Mat Engn, Wenzhou 325035, Zhejiang, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2019年 / 210卷

基金：

中国国家自然科学基金;

关键词：

Split DNAzyme; TMB; Hemin-graphene composites; Colorimetry; T-Hg2+-T; COLORIMETRIC DETECTION; LABEL-FREE; GOLD NANOPARTICLES; ELECTROCHEMICAL APTASENSOR; DNA-DAMAGE; BIOSENSOR; MERCURY; OXIDE; SENSOR; PAIRS;

D O I：

10.1016/j.saa.2018.11.048

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A visual and sensitive Hg2+ detection strategy was developed based on split DNAzyme amplification and hemin-graphene oxide composites (H-GNs). Two split DNAzyme sequences can form two entire enzyme-strands DNA (E-DNA) by T-Hg2+-T interaction. The E-DNA can bind with the loop of molecular beacon (MB) to form Mg2+-dependent DNAzyme structure. The formed DNAzyme can circularly cleave the loop of MB, resulting large amount of DNA fragments. The resultant DNA fragments can prevent H-GNs from aggregation by adsorbing on its surface. Consequently, the supernate with large amount of H-GNs shows dark blue color after chromogenic reaction. This strategy shows a linear range from 50 pM to 1200 pM. The limit detection can be low to 33 pM. This strategy provides a visual and enzyme-free amplification mode for quick and sensitive screen of Hg2+. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：335 / 340

页数：6

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