Au nanoparticles adorned chitosan-modified magnetic nanocomposite: An investigation towards its antioxidant and anti-hepatocarcinoma activity in vitro

被引:26
作者
Ou, Xiancheng [1 ]
Karmakar, Bikash [2 ]
Awwad, Nasser S. [3 ]
Ibrahium, Hala A. [4 ,6 ]
Osman, Hosam-Eldin Hussein [5 ]
El-Kott, Attalla F. [6 ,7 ]
Abdel-Daim, Mohamed M. [8 ,9 ]
机构
[1] Qionghai Peoples Hosp, Dept Gen Surg, Qionghai 571400, Hainan, Peoples R China
[2] Gobardanga Hindu Coll, Dept Chem, 24 Parganas North, Gobardanga, India
[3] King Khalid Univ, Dept Chem, POB 9004, Abha 61413, Saudi Arabia
[4] Nucl Mat Author, Dept Semi Pilot Plant, POB 530, El Maadi, Egypt
[5] Taif Univ, Coll Med, Anat Dept, POB 11099, At Taif 21944, Saudi Arabia
[6] King Khalid Univ, Coll Sci, Biol Dept, Abha 61421, Saudi Arabia
[7] Damanhour Univ, Coll Sci, Zool Dept, Damanhour 22511, Egypt
[8] Batterjee Med Coll, Pharmaceut Sci Dept, Pharm Program, POB 6231, Jeddah 21442, Saudi Arabia
[9] Suez Canal Univ, Fac Vet Med, Pharmacol Dept, Ismailia 41522, Egypt
关键词
Hepatoma; Gold; Chitosan; Magnetic nanocomposite; Antioxidant assay; Liver cancer; HepG2; GOLD NANOPARTICLES; HEPATOCELLULAR-CARCINOMA; PALLADIUM NANOPARTICLES; RECYCLABLE NANOCATALYST; ANTICANCER ACTIVITY; COUPLING REACTIONS; SUZUKI-MIYAURA; CATALYST; REDUCTION; CANCER;
D O I
10.1016/j.inoche.2022.109221
中图分类号
O61 [无机化学];
学科分类号
070301 ; 081704 ;
摘要
Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification and biodegradability. In this study, a green protocol for synthesis of Au nanoparticles (NPs) supported on chitosan-encapsulated magnetic Fe3O4 NP is described. The morphological and physicochemical features of the material were determined using several advanced techniques like Fourier Transformed Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray Spectroscopy (EDS), X-ray Diffraction (XRD), Inductively Coupled Plasma (ICP), Vibrating sample Magnetometer (VSM) and elemental mapping. The average diameter of the NPs was approximately 15-25 nm. In addition, the Fe3O@CS-GA/Au nanocomposite was engaged in biological assays like study of anti-oxidant properties by DPPH mediated free radical scavenging test using BHT as a reference molecule. Thereafter, on having a significant IC50 value in radical scavenging assay, we extended the bio-application of the desired nanocomposite in anticancer study of HepG2 liver cancer cell line in vitro conditions following MTT assay. The cell viability of malignant liver cell line reduced dose-dependently in the presence of Fe3O4@CS-GA/Au nanocomposite. As a result, synthesized stable Fe3O@CS-GA/Au nano composite show more significant anticancer activity against HepG2 cells at 161.25 mu g concentration of NPs without significant toxicity against normal cell line. The recent results suggest that Fe3O4@CS-GA/Au nano composite have a suitable anticancer activity against liver cell lines.
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页数:8
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