Simultaneous quantification of 8 nucleotides and adenosine in cells and their medium using UHPLC-HRMS

被引：3

作者：

Feliu, Catherine ^{[1
]}

Peyret, Helene ^{[1
]}

Vautier, Damien ^{[1
]}

Djerada, Zoubir ^{[1
]}

机构：

[1] Reims Univ Hosp, Dept Pharmacol, SFR CAP Sante, EA 3801, 51 Rue Cognacq Jay, F-51095 Reims, France

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2020年 / 1148卷

关键词：

Nucleotides; Adenosine; Quantification; High-resolution mass spectrometry; Extracellular; Intracellular; POROUS GRAPHITIC CARBON; PERFORMANCE LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; ADENINE-NUCLEOTIDES; QUANTITATIVE-ANALYSIS; EXTRACELLULAR NAADP; CREATINE-PHOSPHATE; STAINLESS-STEEL; NUCLEOSIDES; VALIDATION;

D O I：

10.1016/j.jchromb.2020.122156

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Purinergic signalling is involved in physiological processes, particularly during ischemia-reperfusion injuries for which it has a protective effect. The purpose of this work was to develop a method for simultaneous quantification of eight nucleotides and adenosine in biological matrices by liquid chromatography coupled with high-resolution mass spectrometry. A method was developed that was sufficiently robust to quantify the targeted analytes in 20 min with good sensitivity. Analysis of extracellular media from cultured endothelial cells detected the release of nucleotides and adenosine during 2 h of hypoxia. The quantification of cylic adenosine monophosphate (cAMP) allowed to establish a dose-response curve after receptor stimulation. Therefore, our method allows us to study the involvement of nucleotides in various processes in both the intracellular and extracellular compartment.

引用

页数：14

共 59 条

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