DNA detection with a polymeric nanochannel device

被引：50

作者：

Fanzio, Paola ^{[1
,2
]}

Mussi, Valentina ^{[1
]}

Manneschi, Chiara ^{[1
]}

Angeli, Elena ^{[1
]}

Firpo, Giuseppe ^{[1
]}

Repetto, Luca ^{[1
]}

Valbusa, Ugo ^{[1
]}

机构：

[1] Univ Genoa, Nanomed Labs, Dipartimento Fis, I-16146 Genoa, Italy

[2] Italian Inst Technol, I-16163 Genoa, Italy

来源：

LAB ON A CHIP | 2011年 / 11卷 / 17期

关键词：

SOLID-STATE NANOPORES; TRANSLOCATION; PORE; ION;

D O I：

10.1039/c1lc20243j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present the development and the electrical characterization of a polymeric nanochannel device. Standard microfabrication coupled to Focused Ion Beam (FIB) nanofabrication is used to fabricate a silicon master, which can be then replicated in a polymeric material by soft lithography. Such an elastomeric nanochannel device is used to study DNA translocation events during electrophoresis experiments. Our results demonstrate that an easy and low cost fabrication technique allows creation of a low noise device for single molecule analysis.

引用

页码：2961 / 2966

页数：6

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