Development of a monoclonal antibody for the detection of anti-canine CD20 chimeric antigen receptor expression on canine CD20 chimeric antigen receptor-transduced T cells

被引:4
作者
Sakai, Osamu [1 ]
Ogino, Shoji [2 ]
Tsukui, Toshihiro [2 ]
Igase, Masaya [1 ]
Mizuno, Takuya [1 ]
机构
[1] Yamaguchi Univ, Joint Grad Sch Vet Med, Lab Mol Diagnost & Therapeut, 1677-1 Yoshida, Yamaguchi 7538515, Japan
[2] Nippon Zenyaku Kogyo Co Ltd, Koriyama, Fukushima 9630196, Japan
关键词
adoptive immunotherapy; canine; CD20; chimeric antigen receptorT cell; monoclonal antibody; RITUXIMAB; LYMPHOMA; THERAPY; MODEL; DOGS;
D O I
10.1292/jvms.21-0326
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Chimeric antigen receptor (CAR) CAR-T cell therapy targeting CD20 can be a novel adoptive cell therapy for canine patients with B-cell malignancy. After injection of the CAR-T cells in vivo, monitoring circulating CAR-T cells is essential to prove in vivo persistence of CAR-T cells. In this study, we developed a novel monoclonal antibody against canine CD20 CAR, whose single-chain variable fragment was derived from the our previously reported anti-canine CD20 therapeutic antibody. Furthermore, we proved that this monoclonal antibody can detect therapeutic anti-canine CD20 chimeric antibody in the serum from healthy beagle dogs injected with the therapeutic antibody for safety study. This monoclonal antibody is a useful tool for monitoring both canine CD20-CAR-T cells and anti-canine CD20 therapeutic antibody for canine lymphoma.
引用
收藏
页码:1495 / 1499
页数:5
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