Development of a monoclonal antibody for the detection of anti-canine CD20 chimeric antigen receptor expression on canine CD20 chimeric antigen receptor-transduced T cells

被引：4

作者：

Sakai, Osamu ^{[1
]}

Ogino, Shoji ^{[2
]}

Tsukui, Toshihiro ^{[2
]}

Igase, Masaya ^{[1
]}

Mizuno, Takuya ^{[1
]}

机构：

[1] Yamaguchi Univ, Joint Grad Sch Vet Med, Lab Mol Diagnost & Therapeut, 1677-1 Yoshida, Yamaguchi 7538515, Japan

[2] Nippon Zenyaku Kogyo Co Ltd, Koriyama, Fukushima 9630196, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2021年 / 83卷 / 10期

关键词：

adoptive immunotherapy; canine; CD20; chimeric antigen receptorT cell; monoclonal antibody; RITUXIMAB; LYMPHOMA; THERAPY; MODEL; DOGS;

D O I：

10.1292/jvms.21-0326

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Chimeric antigen receptor (CAR) CAR-T cell therapy targeting CD20 can be a novel adoptive cell therapy for canine patients with B-cell malignancy. After injection of the CAR-T cells in vivo, monitoring circulating CAR-T cells is essential to prove in vivo persistence of CAR-T cells. In this study, we developed a novel monoclonal antibody against canine CD20 CAR, whose single-chain variable fragment was derived from the our previously reported anti-canine CD20 therapeutic antibody. Furthermore, we proved that this monoclonal antibody can detect therapeutic anti-canine CD20 chimeric antibody in the serum from healthy beagle dogs injected with the therapeutic antibody for safety study. This monoclonal antibody is a useful tool for monitoring both canine CD20-CAR-T cells and anti-canine CD20 therapeutic antibody for canine lymphoma.

引用

页码：1495 / 1499

页数：5

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