Simultaneous determination of cis- and trans-boutendioic acid by partial least squares-ultraviolet spectrophotometry

The calibration models were established for the determination of cis- and trans-butendioic acid by partial least squares (PLS) and ultraviolet spectrophotometry. The absorption spectra in the range of 190 268 nm for 23 different mixtures of butendioic acid isomers in phosphoric acid solution (pH = 1.4) were used as calibration set. Correlation coefficients of the models for cis- and trans-butendioic acid were 0.9951 and 0.9983, the mean recoveries were 100.8% and 100.7%, the root mean square errors of prediction (RMSE) were 0.3667 and 0.2233, and the relative error of prediction (REP) were 3.49% and 5.05% in the predicted range of 1.0 - 13.0 and 3..0 - 14.0 mg/L, respectively. By statistical signification test, the results of the determination were compared with those of HPLC without significant difference at 0.05 level. The proposed method has been applied to the simultaneous determination of cis- and trans-butendioic acid in real samples with satisfactory results.