Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism

被引:15
|
作者
Song, Jia [1 ]
Wang, Jun [1 ]
Wang, Xinyu [1 ]
Zhao, Hang [1 ]
Hu, Tao [1 ]
Feng, Zhiwei [1 ]
Lei, Zhi [2 ]
Li, Weizhao [2 ]
Zheng, Yu [1 ]
Wang, Min [1 ]
机构
[1] Tianjin Univ Sci & Technol, Coll Biotechnol, State Key Lab Food Nutr & Safety, Key Lab Ind Fermentat Microbiol,Minist Educ, Tianjin, Peoples R China
[2] Tian Di 1 Beverage Inc, Jiangmen, Peoples R China
基金
中国国家自然科学基金;
关键词
Acetobacter pasteurianus; acetic acid fermentation; transcriptome; energy charge; energy metabolism; STRENGTH VINEGAR FERMENTATION; PLANT SCALE ACETIFIER; RESISTANCE; ACETIFICATION; BACTERIA; GROWTH; ETHANOL; CHARGE; MECHANISMS; TOLERANCE;
D O I
10.3389/fbioe.2022.815614
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Energy metabolism is important for cell growth and tolerance against environment stress. In acetic acid fermentation by Acetobacter pasteurianus, the correlation coefficients of acid production rate with energy charge and ATP content were 0.9981 and 0.9826, respectively. The main energy metabolism pathway, including glycolysis pathway, TCA cycle, ethanol oxidation, pentose phosphate pathway, and ATP production, was constructed by transcriptome analysis. The effects of fermentation conditions, including dissolved oxygen, initial acetic acid concentration, and total concentration, on acetic acid fermentation and energy metabolism of A. pasteurianus were analyzed by using the RT-PCR method. The results showed the high energy charge inhibited glucose catabolism, and associated with the high ethanol oxidation rate. Consequently, a virtuous circle of increased ethanol oxidation, increased energy generation, and acetic acid tolerance was important for improving acetic acid fermentation.
引用
收藏
页数:12
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