Effects of bisphosphonates on alkaline phosphatase activity, mineralization, and prostaglandin E(2) synthesis in the clonal osteoblast-like cell line MC3T3-E1

被引：50
|
作者：
Igarashi, K [1 ]
Hirafuji, M [1 ]
Adachi, H [1 ]
Shinoda, H [1 ]
Mitani, H [1 ]
机构：
[1] TOHOKU UNIV, SCH DENT, DEPT PHARMACOL, AOBA KU, SENDAI, MIYAGI 98077, JAPAN
来源：
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1997年 / 56卷 / 02期
关键词：
D O I：
10.1016/S0952-3278(97)90508-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effects of 3 bisphosphonates, AHBuBP, AHPrBP, and Cl(2)MBP on cell growth, alkaline phosphatase (ALP) activity, mineralization, and prostaglandin E(2) (PGE(2)) synthesis in the clonal osteoblast-like cell line MC3T3-E1 were studied. These bisphosphonates had essentially similar effects on growth and the osteoblastic functions of the cells, i.e., they had no inhibitory effects on cell growth except at higher concentrations, they increased ALP activity, and inhibited PGE(2) production. In the presence of AHBuBP, ALP activity was higher than that in the control after day 6 of culture. Lower concentrations of AHBuBP slightly facilitated mineralization by the cells. It is probable that bisphosphonates enhance the functions of osteoblasts in certain concentration and that the inhibition of endogenous PGE(2) production may be involved in the mechanism of action of bisphosphonates.
引用
收藏
页码：121 / 125
页数：5
相关论文
共 50 条
  • [21] Enhancing effect of daidzein on the differentiation and mineralization in mouse osteoblast-like MC3T3-E1 cells
    Ge, Yuebin
    Chen, Dawei
    Xie, Liping
    Zhang, Rongqing
    YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 2006, 126 (08): : 651 - 656
  • [22] Twisted gastrulation and chordin inhibit differentiation and mineralization in MC3T3-E1 osteoblast-like cells
    Petryk, A
    Shimmi, O
    Jia, XH
    Carlson, AE
    Tervonen, L
    Jarcho, MP
    O'Connor, MB
    Gopalakrishnan, R
    BONE, 2005, 36 (04) : 617 - 626
  • [23] HEPARIN STIMULATES THE COLLAGEN-SYNTHESIS IN MINERALIZED CULTURES OF THE OSTEOBLAST-LIKE CELL-LINE, MC3T3-E1
    SHIBATA, Y
    ABIKO, Y
    GOTO, K
    MORIYA, Y
    TAKIGUCHI, H
    BIOCHEMISTRY INTERNATIONAL, 1992, 28 (02): : 335 - 344
  • [24] Mitogenic Action of Adenosine on Osteoblast-Like Cells, MC3T3-E1
    S. Shimegi
    Calcified Tissue International, 1998, 62 : 418 - 425
  • [25] Mitogenic action of adenosine on osteoblast-like cells, MC3T3-E1
    Shimegi, S
    CALCIFIED TISSUE INTERNATIONAL, 1998, 62 (05) : 418 - 425
  • [26] Phenotypic heterogeneity of osteoblast-like MC3T3-E1 cells: Changes of bradykinin-induced prostaglandin E(2) production during osteoblast maturation
    Leis, HJ
    Hulla, W
    Gruber, R
    Huber, E
    Zach, D
    Gleispach, H
    Windischhofer, W
    JOURNAL OF BONE AND MINERAL RESEARCH, 1997, 12 (04) : 541 - 551
  • [27] Tyrosine phosphorylation in osteoblast-like MC3T3-E1 cell in response O carcinoma cell line supernatants.
    Hamilton, G
    Ulsperger, E
    Haberl, I
    Raderer, M
    Hoffmann, O
    BRITISH JOURNAL OF CANCER, 1999, 80 : 72 - 72
  • [28] STIMULATION BY BONE SIALOPROTEIN OF CALCIFICATION IN OSTEOBLAST-LIKE MC3T3-E1 CELLS
    ZHOU, HY
    TAKITA, H
    FUJISAWA, R
    MIZUNO, M
    KUBOKI, Y
    CALCIFIED TISSUE INTERNATIONAL, 1995, 56 (05) : 403 - 407
  • [29] Role of Sandhika: A polyherbal formulation on MC3T3-E1 osteoblast-like cells
    Tripathi, Yamini B.
    Tripathi, Pratibha
    Korlagunta, Kiranmayi
    Chai, Sheau Ching
    Smith, Brenda J.
    Arjmandi, Bahram H.
    INFLAMMATION, 2008, 31 (01) : 1 - 8
  • [30] Panax notoginseng stimulates alkaline phosphatase activity, collagen synthesis, and mineralization in osteoblastic MC3T3-E1 cells
    Zhe Ji
    Yizhao Cheng
    Puwei Yuan
    Xiaoqian Dang
    Xiong Guo
    Weizhuo Wang
    In Vitro Cellular & Developmental Biology - Animal, 2015, 51 : 950 - 957
12345