Non-coding RNA LOXL1-AS1 exhibits oncogenic activity in ovarian cancer via regulation of miR-18b-5p/VMA21 axis

被引：34
作者：
Xue, Fang [1 ]
Xu, Yan Hua [1 ]
Shen, Cheng Cheng [1 ]
Qin, Zhen Li [1 ]
Zhou, Hai Bin [2 ]
机构：
[1] Jinan Maternal & Child Hlth Hosp, Dept Gynaecol & Obstet, Jinan 250012, Shandong, Peoples R China
[2] Shandong Univ, Qilu Hosp, Dept Gynaecol & Obstet, Infertil Ctr, Jinan 250012, Shandong, Peoples R China
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2020年 / 125卷
关键词：
LOXL1-AS1; Ovarian cancer; Migration; Invasion; miR-18b-5p; CELL LUNG-CANCER; PROMOTES APOPTOSIS; INVASION; PROLIFERATION; METASTASIS; EXPRESSION;
D O I：
10.1016/j.biopha.2019.109568
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Long non-coding RNAs (lncRNAs) exert critical effects in the process of malignant cancers and lncRNA LOXL1 Antisense RNA 1 (LOXL1-AS1) has been demonstrated to be a pro-oncogene in multiple tumor types. In the current study, we illuminated the precise roles of LOXL1-AS1 in the development of ovarian cancer. LOXL1-AS1 is significantly overexpressed in ovarian carcinoma tissue compared with adjacent non-cancerous sample. The luciferase reporter gene assay reveals the relationship between LOXL1-AS1 and miR-18b-5p, miR-18b-5p and its target gene, Vacuolar ATPase Assembly Factor VMA21 (VMA21). Transfection of LOXL1-AS1 siRNA or miR-18b-5p mimics inhibits the growth and aggressive phenotypes of SKOV3 and OVCAR3 cell. Furthermore, miR-18b-5p suppresses ovarian carcinoma cell proliferation and metastasis by targeting VMA21 and LOXL1-AS1 regulates ovarian carcinoma cell growth and metastasis through sponging miR-18b-5p. These findings suggest that lncRNA LOXL1-AS1 promotes ovarian cancer cell growth, migratory and invasiveness via modulating miR-18b-5p/VMA21 axis.
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页数：11
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