Interactions of Cytochrome c with DNA at glassy carbon surface

被引：15

作者：

Bi, YH ^{[1
]}

Huang, ZL ^{[1
]}

Zhao, YD ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Hubei Bioinformat & Mol Imaging Key Lab, Key Lab Biomed Photon, Minist Educ, Wuhan 430074, Hubei, Peoples R China

来源：

BIOPHYSICAL CHEMISTRY | 2005年 / 116卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Cytochrome c; interaction; DNA; modified electrode;

D O I：

10.1016/j.bpc.2005.03.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyclic voltammetry (CV) was used to investigate the interactions of Cytochrome c (Cyt c) with deoxyribonucleic acid (DNA) at glassy carbon (GC) electrodes. The results indicate that there are strong interactions between Cyt c and DNA. The binding constant (kA) and binding free energy (Delta(r)G) of Cyt c with dsDNA are (1.69 +/- 0.38) x 10(5) L-mol(-1) and -(29.76 +/- 0.56)kJ.mol(-1), respectively; and those of Cyt c with ssDNA are (3.35 +/- 0.50) x 10(5) L-mol(-1) and -(31.49 +/- 0.37) kJ-mol(-1), respectively. The binding sites are achieved to be 3.3 bp per Cyt c molecule with dsDNA and 4.0 nucleotides (ssDNA) binding one Cyt c molecule. This experiment affords a valid method for investigating the interactions between DNA and proteins by electrochemical techniques. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：193 / 198

页数：6

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