Total Flavonoids from Oroxylum indicum Induce Apoptosis via PI3K/Akt/PTEN Signaling Pathway in Liver Cancer

被引：16
作者：
Li, Nan-nan [1 ]
Meng, Xian-sheng [1 ,2 ,3 ]
Men, Wen-xiao [1 ]
Bao, Yong-rui [1 ,2 ,3 ]
Wang, Shuai [1 ,2 ,3 ]
机构：
[1] Liaoning Univ Tradit Chinese Med, Sch Pharm, Dalian 116600, Peoples R China
[2] Component Med Engn Res Ctr Liaoning Prov, Dalian 116600, Peoples R China
[3] Liaoning Prov Modern Chinese Med Res Engn Lab, Dalian 116600, Peoples R China
来源：
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2018年 / 2018卷
关键词：
HEPATOCELLULAR-CARCINOMA; IN-VITRO; CELL; PROLIFERATION; ANTIOXIDANT; ACTIVATION; MICE; VIVO;
D O I：
10.1155/2018/3021476
中图分类号：
R [医药、卫生];
学科分类号：
10 ;
摘要：
Total flavonoids (TF), derived from the seeds of Oroxylum indicum (L.) Vent., possess many pharmacological functions. In the present study, H-22 - bearing mice and SMMC-7721 models were employed to evaluate the antitumor activity of TF and to and investigate its possible mechanisms both in vitro and in vivo. Cell viability was evaluated by MTT assay; cell apoptosis rate was analyzed via Annexin V-FITC/PI double staining by flow cytometer. Meanwhile, the expressions of apoptosis-related mRNA and proteins were evaluated by RT-PCR and Western blot analysis. The results revealed that TF could significantly inhibit the tumor growth, and the possible mechanism was related to the effect of inducing tumor cells apoptosis through PI3K/Akt/PTEN signaling pathway. This study has provided a theoretical basis for the further development and application of TF as antitumor drugs.
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页数：9
相关论文
共 34 条
[1]   Gastroprotective flavonoid constituents from Oroxylum indicum Vent. [J].
Babu, T. Hari ;
Manjulatha, K. ;
Kumar, G. Suresh ;
Hymavathi, A. ;
Tiwari, Ashok K. ;
Purohit, Muraleedhar ;
Rao, J. Madhusudana ;
Babu, K. Suresh .
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2010, 20 (01) :117-120
[2]   A comparison of trends in mortality from primary liver cancer and intrahepatic cholangiocarcinoma in Europe [J].
Bertuccio, P. ;
Bosetti, C. ;
Levi, F. ;
Decarli, A. ;
Negri, E. ;
La Vecchia, C. .
ANNALS OF ONCOLOGY, 2013, 24 (06) :1667-1674
[3]   Accelerated therapeutic progress in diffuse large B cell lymphoma [J].
Cai, Qingqing ;
Westin, Jason ;
Fu, Kai ;
Desai, Madhav ;
Zhang, Liang ;
Huang, Huiqiang ;
Jiang, Wenqi ;
Liang, Rong ;
Qian, Zhengzi ;
Champlin, Richard E. ;
Wang, Michael .
ANNALS OF HEMATOLOGY, 2014, 93 (04) :541-556
[4]  
Caleb K. F., 2016, PHARM RES, V110, P101, DOI DOI 10.HTTP://DX.D0I.0RG/10.1016/J
[5]   Induction of Apoptosis in HepaRG Cell Line by Aloe-Emodin through Generation of Reactive Oxygen Species and the Mitochondrial Pathway [J].
Dong, Xiaoxv ;
Fu, Jing ;
Yin, Xingbin ;
Qu, Changhai ;
Yang, Chunjing ;
He, Huyiligeqi ;
Ni, Jian .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2017, 42 (02) :685-696
[6]   Diabetes increases the risk of chronic liver disease and hepatocellular carcinoma [J].
El-Serag, HB ;
Tran, T ;
Everhart, JE .
GASTROENTEROLOGY, 2004, 126 (02) :460-468
[7]   Apoptosis: a basic biological phenomenon with wide-ranging implications in human disease [J].
Fadeel, B ;
Orrenius, S .
JOURNAL OF INTERNAL MEDICINE, 2005, 258 (06) :479-517
[8]   Morusin shows potent antitumor activity for human hepatocellular carcinoma in vitro and in vivo through apoptosis induction and angiogenesis inhibition [J].
Gao, Ling ;
Wang, Li ;
Sun, Zhen ;
Li, Haiyan ;
Wang, Qiaoping ;
Yi, Cheng ;
Wang, Xiujie .
DRUG DESIGN DEVELOPMENT AND THERAPY, 2017, 11 :1789-1802
[9]   Activation of hepatic stellate cell in Pten null liver injury model [J].
He, Lina ;
Gubbins, James ;
Peng, Zhechu ;
Medina, Vivian ;
Fei, Fan ;
Asahina, Kinji ;
Wang, Jiaohong ;
Kahn, Michael ;
Rountree, Carl B. ;
Stiles, Bangyan L. .
FIBROGENESIS & TISSUE REPAIR, 2016, 9
[10]   Thymoquinone: An IRAK1 inhibitor with in vivo and in vitro anti-inflammatory activities [J].
Hossen, Muhammad Jahangir ;
Yang, Woo Seok ;
Kim, Daewon ;
Aravinthan, Adithan ;
Kim, Jong-Hoon ;
Cho, Jae Youl .
SCIENTIFIC REPORTS, 2017, 7
1234