White clover mosaic virus-induced gene silencing in pea

被引：14
|
作者：
Ido, Yukari [1 ]
Nakahara, Kenji S. [1 ]
Uyeda, Ichiro [1 ]
机构：
[1] Hokkaido Univ, Res Fac Agr, Pathogen Plant Interact Grp, Sapporo, Hokkaido 0608589, Japan
来源：
JOURNAL OF GENERAL PLANT PATHOLOGY | 2012年 / 78卷 / 02期
关键词：
Pea; RNA silencing; Potexvirus; NUCLEOTIDE-SEQUENCE; VECTORS; PROTECTION; PROTEIN; TOBACCO;
D O I：
10.1007/s10327-012-0360-3
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Double-stranded RNAs formed in secondary structures and replicative intermediates of viral genomes are thought to strongly elicit RNA silencing. This phenomenon is known as virus-induced gene silencing (VIGS). VIGS is a powerful tool for modifying gene expression in host plants. We constructed a virus vector based on White clover mosaic virus (WC1MV) and demonstrated VIGS of phytoene desaturase (PDS) in pea. Photobleaching of tissues, caused by VIGS of PDS, was observed in restricted areas of upper leaves and stems. We confirmed that the PDS mRNA and subgenomic RNAs of WC1MV were reduced in the photobleached tissues.
引用
收藏
页码：127 / 132
页数：6
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