Toxoplasma gondii: Protective immunity against toxoplasmosis with recombinant actin depolymerizing factor protein in BALB/c mice

被引：21

作者：

Huang, Xiangsheng ^{[1
]}

Li, Jianhua ^{[1
]}

Zhang, Guocai ^{[1
]}

Gong, Pengtao ^{[1
]}

Yang, Ju ^{[1
]}

Zhang, Xichen ^{[1
]}

机构：

[1] Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Peoples R China

来源：

EXPERIMENTAL PARASITOLOGY | 2012年 / 130卷 / 03期

关键词：

Toxoplasma gondii; Recombinant protein; Actin depolymerizing factor; BALB/c mice; VACCINE CANDIDATE; INFECTION; ANTIGEN; GENE; IMMUNIZATION; CYTOSKELETON; COMBINATION; MOTILITY; INVASION; PROTOZOA;

D O I：

10.1016/j.exppara.2012.01.001

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Toxoplasmosis is one of the most world-wide spread zoonosis representing a very serious clinical and veterinary problem. There is still need for vaccines for toxoplasmosis. In the present study, we evaluated the protective efficacy of a recombinant actin depolymerizing factor (ADF) subunit vaccine against Toxoplasma gondii infection in BALB/c mice. The recombinant T. gondii ADF protein (rADF) was expressed in Escherichia coli and used as antigens for BALB/c mice immunization. The results indicated that specific antibody and the increased percentage of CD4(+) T lymphocyte were found in vaccinated BALB/c mice with rADF, when compared with adjuvant or PBS groups. After challenged with T. gondii (RH strain) tachyzoites, the survival time of the mice in rADF group was longer than those in the control group. The numbers of brain cysts of the mice in rADF group reduced significantly when compared with those in control groups (P < 0.05), and the rate of reduction could reach to around 30%. These results suggest that rADF can generate protective immunity against T. gondii infection in BALB/c mice. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：218 / 222

页数：5

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