Highly sensitive fluorescent detection of trypsin based on BSA-stabilized gold nanoclusters

被引：230
作者：
Hu, Lianzhe [1 ,2 ]
Han, Shuang [1 ,2 ]
Parveen, Saima [1 ,2 ,3 ]
Yuan, Yali [1 ,2 ]
Zhang, Ling [1 ,2 ]
Xu, Guobao [1 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Beijing 100864, Peoples R China
[3] Islamia Univ Bahawalpur, Dept Chem, Fac Sci, Bahawalpur 63100, Pakistan
来源：
BIOSENSORS & BIOELECTRONICS | 2012年 / 32卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Fluorescence; Nanoclusters; Biosensors; Gold; Protease; AU NANOCLUSTERS; PROTEASE; ASSAY; NANOPARTICLES; CLUSTERS; PROBE; WATER; HG2+;
D O I：
10.1016/j.bios.2011.12.007
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In this study, fluorescent metal nanoclusters are presented as novel probes for sensitive detection of protease for the first time. The sensing mechanism is based on trypsin digestion of the protein template of BSA-stabilized Au nanoclusters. The decrease in fluorescence intensity of BSA-Au nanoclusters caused by trypsin allows the sensitive detection of trypsin in the range of 0.01-100 mu g/mL. The detection limit for trypsin is 2 ng/mL (86 pM) at a signal-to-noise ratio of 3. The present nanosensor for trypsin detection possesses red emission, excellent biocompatibility, high selectivity, and good stability. In addition, we demonstrated the application of the present approach in real urine samples, which suggested its potential for diagnostic purposes. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：297 / 299
页数：3
相关论文
共 28 条
[1]   Electronic transduction of HIV-1 drug resistance in AIDS patients [J].
Alfonta, L ;
Blumenzweig, I ;
Zayats, M ;
Baraz, L ;
Kotler, M ;
Willner, I .
CHEMBIOCHEM, 2004, 5 (07) :949-957
[2]   Label-Free, Homogeneous, and Fluorescence "Turn-On" Detection of Protease Using Conjugated Polyelectrolytes [J].
An, Lingling ;
Liu, Libing ;
Wang, Shu .
BIOMACROMOLECULES, 2009, 10 (02) :454-457
[3]   Etching colloidal gold nanocrystals with hyperbranched and multivalent polymers: A new route to fluorescent and water-soluble atomic clusters [J].
Duan, Hongwei ;
Nie, Shuming .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (09) :2412-+
[4]   Development of a protease biosensor utilizing silica nanobeads [J].
Grant, Sheila A. ;
Weilbaecher, Craig ;
Lichlyter, Darcy .
SENSORS AND ACTUATORS B-CHEMICAL, 2007, 121 (02) :482-489
[5]   Relationship between protein structure and methionine oxidation in recombinant human α1-antitrypsin [J].
Griffiths, SW ;
Cooney, CL .
BIOCHEMISTRY, 2002, 41 (20) :6245-6252
[6]   Gold nanoparticles-based protease assay [J].
Guarise, C ;
Pasquato, L ;
De Filippis, V ;
Scrimin, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (11) :3978-3982
[7]   Fluorescent Ag Clusters via a Protein-Directed Approach as a Hg(II) Ion Sensor [J].
Guo, Cunlan ;
Irudayaraj, Joseph .
ANALYTICAL CHEMISTRY, 2011, 83 (08) :2883-2889
[8]   Oligonucleotide-stabilized Ag nanoclusters as novel fluorescence probes for the highly selective and sensitive detection of the Hg2+ ion [J].
Guo, Weiwei ;
Yuan, Jipei ;
Wang, Erkang .
CHEMICAL COMMUNICATIONS, 2009, (23) :3395-3397
[9]   Synthesis of highly fluorescent gold nanoparticles for sensing Mercury(II) [J].
Huang, Chih-Ching ;
Yang, Zusing ;
Lee, Kun-Hong ;
Chang, Huan-Tsung .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (36) :6824-6828
[10]   Biomolecule-stabilized Au nanoclusters as a fluorescence probe for sensitive detection of glucose [J].
Jin, Lihua ;
Shang, Li ;
Guo, Shaojun ;
Fang, Youxing ;
Wen, Dan ;
Wang, Li ;
Yin, Jianyuan ;
Dong, Shaojun .
BIOSENSORS & BIOELECTRONICS, 2011, 26 (05) :1965-1969
123