Detection of bovine viral diarrhoea virus (BVDV) nucleic acid and antigen in different organs of water buffaloes (Bubalus bubalis)

被引：15

作者：

Craig, M. I. ^{[1
]}

Venzano, A. ^{[2
]}

Konig, G.

Morris, W. E. ^{[2
]}

Jimenez, L. ^{[1
]}

Julia, S. ^{[1
]}

Capellino, F. ^{[2
]}

Viera, J. Blanco ^{[2
]}

Weber, E. L. ^{[1
]}

机构：

[1] INTA, Ctr Invest Ciencias Vet & Agron, Inst Virol, RA-1712 Buenos Aires, DF, Argentina

[2] INTA, Ctr Invest Ciencias Vet & Agron, Inst Patobiol, RA-1712 Buenos Aires, DF, Argentina

来源：

RESEARCH IN VETERINARY SCIENCE | 2008年 / 85卷 / 01期

关键词：

pestivirus; BVDV; buffaloes; immunohistochemistry; RT-PCR;

D O I：

10.1016/j.rvsc.2007.08.013

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Bovine viral diarrhoea virus (BVDV) is a pestivirus that infects mainly bovine cattle. Nevertheless, there are several reports about infections in other members of the Artiodactyla order including serological studies, that indicate infection of BVDV in buffaloes. The aim of this article is to study the presence of BVDV in three young water buffaloes, displaying nonspecific clinical signs, compatible with the BVDV infection. Both immunohistochemistry and RT-PCR confirmed the presence of BVDV in the animals. The sequence analysis on RT-PCR amplicons revealed high identity with reference strains of genotypes 1a and 1b. Although BVDV was unequivocally identified in the sick animals, it has not been proved it is responsible for the clinical signs. Further studies are needed to clarify the pathogenic role of BVDV infection in this animal species, and the role of buffaloes in the epidemiology of BVDV infection. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：194 / 196

页数：3

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