Transcriptome Analysis of Bacillus licheniformis for Improving Bacitracin Production

被引：14
作者：
Wu, Zhiyong [1 ,2 ,3 ]
Li, Youran [1 ,2 ,3 ]
Xu, Yinbiao [1 ,2 ,3 ]
Zhang, Yupeng [1 ,2 ,3 ]
Tao, Guanjun [4 ]
Zhang, Liang [1 ,2 ,3 ]
Shi, Guiyang [1 ,2 ,3 ]
机构：
[1] Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Wuxi 214122, Jiangsu, Peoples R China
[2] Jiangnan Univ, Natl Engn Res Ctr Cereal Fermentat & Food Biomfg, Wuxi 214122, Jiangsu, Peoples R China
[3] Jiangnan Univ, Jiangsu Prov Engn Res Ctr Bioact Prod Proc, Wuxi 214122, Jiangsu, Peoples R China
[4] Jiangnan Univ, Synerget Innovat Ctr Food Safety & Nutr, Collaborat Innovat Ctr Food Safety & Qual Control, State Key Lab Food Sci & Technol,Sch Food Sci & T, Wuxi 214122, Jiangsu, Peoples R China
来源：
ACS SYNTHETIC BIOLOGY | 2022年 / 11卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Bacillus licheniformis; bacitracin; transcriptome; type II thioesterase; dissolved oxygen; VITREOSCILLA HEMOGLOBIN; EXPRESSION; BIOSYNTHESIS; GENE; PROMOTER; SUBTILIS; OPERON;
D O I：
10.1021/acssynbio.1c00593
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This study aims to find the targets that may influence the production of bacitracin based on RNA sequencing in Bacillus licheniformis. Transcriptional profiling revealed that (i) the expression of the bacT gene, encoding a type II thioesterase (TEIIbac) was positively correlated with bacitracin production and (ii) the oxygen uptake exhibited significant influence on precursor synthesis. The verified experiments showed that the overexpression of TEIIbac with an endogenous promoter increased the bacitracin A titer by 37.50%. Furthermore, the increase of oxygen availability through Vitreoscilla hemoglobin (VHb) expression increased the bacitracin A titer by 126.67% under oxygen-restricted conditions. From the transcriptome perspective, the results of this paper demonstrate that TEIIbac and oxygen supply are crucial to bacitracin production. This study also provides insights into the construction of chassis cells for the industrial production of secondary metabolites with a preference for aerobic conditions.
引用
收藏
页码：1325 / 1335
页数：11
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