Conclusive remarks. Reliability and comparability of chlorophyll fluorescence data from several field teams

被引：24

作者：

Bussotti, Filippo ^{[1
]}

Pollastrini, Martina ^{[1
]}

Cascio, Chiara ^{[1
]}

Desotgiu, Rosanna ^{[1
]}

Gerosa, Giacomo ^{[2
]}

Marzuoli, Riccardo ^{[2
]}

Nali, Cristina ^{[3
]}

Lorenzini, Giacomo ^{[3
]}

Pellegrini, Elisa ^{[3
]}

Carucci, Maria Giovanna ^{[3
]}

Salvatori, Elisabetta ^{[4
]}

Fusaro, Lina ^{[4
]}

Piccotto, Massimo ^{[5
]}

Malaspina, Paola ^{[6
]}

Manfredi, Alice ^{[6
]}

Roccotello, Enrica ^{[6
]}

Toscano, Stefania ^{[7
]}

Gottardini, Elena ^{[8
]}

Cristofori, Antonella ^{[8
]}

Fini, Alessio ^{[9
]}

Weber, Daniel ^{[10
]}

Baldassarre, Valentina ^{[11
]}

Barbanti, Lorenzo ^{[12
]}

Monti, Andrea ^{[12
]}

Strasser, Reto J. ^{[13
]}

机构：

[1] Univ Florence, Dept Agr Biotechnol, I-50144 Florence, Italy

[2] Catholic Univ Brescia, Dept Math & Phys, Brescia, Italy

[3] Univ Pisa, Dept Tree Sci Entomol & Plant Pathol Giovanni Sca, Pisa, Italy

[4] Univ Roma La Sapienza, Dept Plant Biol, Rome, Italy

[5] Univ Trieste, Dept Life Sci, Trieste, Italy

[6] Univ Genoa, DIPTERIS, Polo Bot Hanbury, Italy

[7] Univ Catania, Fac Agr, I-95124 Catania, Italy

[8] Fdn Edmund Mach, IASMA Res & Innovat Ctr, San Michele All Adige, Trento, Italy

[9] Univ Florence, Dept Hort, I-50144 Florence, Italy

[10] Biodivers & Climate Res Ctr, Frankfurt, Germany

[11] Univ Milan, Dept Plant Prod, I-20122 Milan, Italy

[12] Univ Bologna, Dept Agroenvironm Sci & Technol, I-40126 Bologna, Italy

[13] Univ Geneva, Dept Plant Biol, CH-1211 Geneva 4, Switzerland

来源：

ENVIRONMENTAL AND EXPERIMENTAL BOTANY | 2011年 / 73卷

关键词：

Chlorophyll fluorescence; Field exercises; F-V/F-M; Harmonization; Intercalibration; SPATIOTEMPORAL VARIABILITY;

D O I：

10.1016/j.envexpbot.2010.10.023

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Two field exercises were carried out to compare chlorophyll a fluorescence measurements taken in the field by field teams working on the same project. In the first exercise (2007, Passo Pura, Ampezzo, Udine, Northern Italy) the operators took measurements on the same leaf areas (maintaining fixed leaf clips); in the second (2009, Monterotondo Marittimo, Grosseto, Central Italy) the teams worked independently, but addressing a common research question. The results of the first exercise showed that: (a) F-V/F-M was stable and had little variation among teams and instruments; (b) the results from the different teams correlated well; (c) the most suitable parameters of fast kinetics analysis are those measured on the normalized fluorescence transients. In the second exercise, when the teams worked independently, the results were much more variable and the correlations between measurements of different operators were weak. These results suggest that field chlorophyll a fluorescence measurements taken by different teams/operators can be comparable only if particular care is taken to the internal variability of the samples and a standardized sampling strategy is applied. A statistically sound representation of a population can be then reached. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：116 / 119

页数：4

共 11 条

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