Organic osmolyte transport in quiescent and activated rat hepatic stellate cells (Ito cells)

被引:38
作者
Peters-Regehr, T [1 ]
Bode, JG [1 ]
Kubitz, R [1 ]
Häussinger, D [1 ]
机构
[1] Univ Dusseldorf, Med Klin, Klin Gastroenterol Hepatol & Infektiol, D-40225 Dusseldorf, Germany
关键词
D O I
10.1002/hep.510290111
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Activation of hepatic stellate cells (HSCs) results in multiple alterations of cell function, but nothing is known about organic osmolytes in these cells. Organic osmolyte transport and transporter messenger RNA (mRNA) expression was studied in quiescent rat HSCs and after their transformation into alpha(1)-smooth muscle actin-positive myofibroblastlike cells, Quiescent stellate cells expressed in an osmosensitive manner the mRNA levels of the transporters for taurine (TAUT) and myoinositol (SMIT), whereas that for betaine was not detectable. However, these cells showed osmosensitive uptake not only of taurine and myoinositol but also of betaine. Osmosensitive betaine uptake was mediated by amino acid transport system A. After transformation into myofibroblasts, taurine and myoinositol uptake increased 5.5-fold and 4.5-fold, respectively, together with the respective transporter mRNA. levels. Betaine uptake increased twofold because of osmosensitive induction of BGT1 expression. In both quiescent and activated HSCs, hypoosmotic cell swelling induced a rapid and 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid-sensitive osmolyte efflux, In quiescent HSCs, hyperosmotic exposure increased the messenger RNA (mRNA) level of cyclooxygenase-2, which was counteracted by taurine but not by betaine or myoinositol, The study identifies taurine, myoinositol, and betaine as osmolytes in HSCs. Transformation of HSCs is accompanied by enhanced osmolyte transport activity and induction of the BGT1 transporter, which may be another activation marker of HSCs.
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页码:173 / 180
页数:8
相关论文
共 50 条
[1]   THE ANTIOXIDANT ACTION OF TAURINE, HYPOTAURINE AND THEIR METABOLIC PRECURSORS [J].
ARUOMA, OI ;
HALLIWELL, B ;
HOEY, BM ;
BUTLER, J .
BIOCHEMICAL JOURNAL, 1988, 256 (01) :251-255
[2]  
BIANCHIN L, 1992, INTERACTION CELL VOL, P249
[3]   MOLECULAR-BASIS FOR OSMOREGULATION OF ORGANIC OSMOLYTES IN RENAL MEDULLARY CELLS [J].
BURG, MB .
JOURNAL OF EXPERIMENTAL ZOOLOGY, 1994, 268 (02) :171-175
[4]   ANISOSMOTIC CELL-VOLUME REGULATION - A COMPARATIVE VIEW [J].
CHAMBERLIN, ME ;
STRANGE, K .
AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 257 (02) :C159-C173
[5]  
FRIEDMAN SL, 1993, NEW ENGL J MED, V328, P1828
[6]  
GRAF J, 1992, INTERACTION CELL VOL, P67
[7]   QUANTITATIVE PROTON MAGNETIC-RESONANCE OF PLASMA FROM UREMIC PATIENTS DURING DIALYSIS [J].
GRASDALEN, H ;
BELTON, PS ;
PRYOR, JS ;
RICH, GT .
MAGNETIC RESONANCE IN CHEMISTRY, 1987, 25 (09) :811-816
[8]   CELLULAR SOURCES OF NONCOLLAGENOUS MATRIX PROTEINS - ROLE OF FAT-STORING CELLS IN FIBROGENESIS [J].
GRESSNER, AM ;
BACHEM, MG .
SEMINARS IN LIVER DISEASE, 1990, 10 (01) :30-46
[9]  
GRESSNER AM, 1995, J HEPATOL, V22, P28
[10]   SYNERGISM BETWEEN HEPATOCYTES AND KUPFFER CELLS IN THE ACTIVATION OF FAT-STORING CELLS (PERISINUSOIDAL LIPOCYTES) [J].
GRESSNER, AM ;
LOTFI, S ;
GRESSNER, G ;
HALTNER, E ;
KROPF, J .
JOURNAL OF HEPATOLOGY, 1993, 19 (01) :117-132