α-Galactosidases production by Debaryomyces hansenii UFV-1

被引：9

作者：

Viana, Pollyanna Amaral ^{[1
,2
]}

de Rezende, Sebastiao Tavares ^{[1
]}

Lopes Passos, Flavia Maria ^{[1
]}

Machado, Solimar Goncalves ^{[1
]}

Maitan, Gabriela Picollo ^{[1
]}

da Silva Coelho, Vinicio Tadeu ^{[3
]}

Guimaraes, Valeria Monteze ^{[1
]}

机构：

[1] Univ Fed Vicosa, BIOAGRO, BR-36570000 Vicosa, MG, Brazil

[2] Univ Fed Vicosa, BR-35690000 Florestal, MG, Brazil

[3] Univ Fed Minas Gerais, Dept Bioquim & Imunol ICB, BR-31270901 Belo Horizonte, MG, Brazil

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2011年 / 20卷 / 03期

关键词：

Debaryomyces hansenii UFV-1; alpha-galactosidase; culture media; carbon source; enzyme production; HYDROLYSIS; OLIGOSACCHARIDES; TRANSPORTER; PROTEINS; WOOD;

D O I：

10.1007/s10068-011-0085-7

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Extracellular and intracellular alpha-galactosidases were produced by yeast Debaryomyces hansenii UFV-1 grown on different media with several carbon sources. D. hansenii grown in YP-medium (1% yeast extract and 2% peptone) presented maximum cell mass (8.45 mg/mL) after 36 h of cultivation, with lactose as carbon source, followed by sucrose, glucose, raffinose, and galactose. Higher extracellular and intracellular alpha-galactosidases activities were observed at 48 h of D. hansenii cultivation in YPmedium containing galactose (0.97 and 5.27 U/mL) and lactose (1.28 and 4.88 U/mL), supporting the evidence for the model of induction for the yeast GAL/MEL regulon, such as described in Sacharomyces cereviseae.

引用

页码：601 / 606

页数：6

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