The Equilibrium of Phosphatidylcholine-Amino Acid System in Monolayer at the Air/water Interface

被引：11

作者：

Petelska, Aneta D. ^{[1
]}

Naumowicz, Monika ^{[1
]}

Figaszewski, Zbigniew A. ^{[1
,2
]}

机构：

[1] Univ Bialystok, Inst Chem, PL-15443 Bialystok, Poland

[2] Univ Warsaw, Fac Chem, Lab Electrochem Power Sources, PL-02093 Warsaw, Poland

来源：

CELL BIOCHEMISTRY AND BIOPHYSICS | 2011年 / 60卷 / 03期

关键词：

Phosphatidylcholine; Tyrosine; Phenylalanine; Complex formation equilibrium; Monolayer; Langmuir trough; OCTADECANOYL-L-ALANINE; LANGMUIR-BLODGETT-FILMS; FTIR SPECTROSCOPY; LB FILMS; SURFACTANTS; CHOLESTEROL; DISCRIMINATION; CONFORMATION; AMPHIPHILES; TYROSINE;

D O I：

10.1007/s12013-010-9133-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monolayers of phosphatidylcholine, tyrosine, and phenylalanine and binary mixtures phosphatidylcholine-tyrosine or phosphatidylcholine-phenylalanine were investigated at the air/water interface. Phosphatidylcholine (lecithin, PC), tyrosine (Tyr), and phenylalanine (Phe) were used in the experiment. The surface tension values of pure and mixed monolayers were used to calculate pi-A isotherms. The surface tension measurements were carried out at 22A degrees C using an improved Teflon trough and a Nima 9000 tensiometer. The Teflon trough was filled with a subphase of triple-distilled water. Known amounts of lipid dissolved in 1-chloropropane were placed at the surface using a syringe. The interactions between lecithin and amino acid result in significant deviations from the additivity rule. An equilibrium theory to describe the behavior of monolayer components at the air/water interface was developed in order to obtain the stability constants of PC-Tyr as well as PC-Phe complexes. We considered the equilibrium between the individual components and the complex and established that lecithin and amino acid formed highly stable 1:1 complex.

引用

页码：155 / 160

页数：6

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