Target catalyzed hairpin assembly for constructing a ratiometric electrochemical aptasensor

被引:55
作者
Gao, Fenglei [1 ]
Qian, Yong [2 ]
Zhang, Lei [2 ]
Dai, Shizhen [2 ]
Lan, Yanfei [2 ]
Zhang, Yu [1 ]
Du, Lili [1 ]
Tang, Daoquan [1 ]
机构
[1] Xuzhou Med Coll, Sch Pharm, Jiangsu Key Lab New Drug Res & Clin Pharm, Xuzhou 221004, Peoples R China
[2] E China Inst Technol, Fundamental Sci Radioact Geol & Explorat Technol, Nanchang 330013, Jiangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Ratiometric; Thrombin; Electrochemical; Aptasensor; HIGHLY SENSITIVE DETECTION; SURFACE-PLASMON RESONANCE; ASSISTED SIGNAL AMPLIFICATION; ROLLING CIRCLE AMPLIFICATION; LABEL-FREE APTASENSOR; ULTRASENSITIVE DETECTION; THROMBIN DETECTION; SMALL-MOLECULE; FLUORESCENT DETECTION; SILVER NANOCLUSTERS;
D O I
10.1016/j.bios.2015.04.040
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In this paper, we develop a novel dual-signaling amplified aptasensor for protein detection via target-catalyzed hairpin assembly. Thrombin was chosen as a model target. This aptasensor contains two DNA hairpins termed as H1 and H2. H1, which is modified at its 3' ends with a methylene blue (MB), consists of the aptamer sequence of human thrombin. Meanwhile, H2 which is modified at its 3' ends with a ferrocene (Fc), is partially complementary to H1. Upon the addition of target protein, it can facilitate the opening of the hairpin structure of H1 and thus accelerate the hybridization between H1 and H2, the target protein can be displaced from hairpin H1 by hairpin H2 through a process similar to DNA branch migration. The released target found another H1 to trigger the cycle, resulting in the multiplication of the Fc confined near the GE surface and MB away from the GE surface. When I-Fc/I-MB is used as the response signal for quantitative determination of thrombin, the detection limit (41 fM) is much lower than that by using either MB or Fc alone. This new dual-signaling aptasensor is readily regenerated and shows good response toward the target. Furthermore, this amplified aptasensor shows high selectivity toward its target protein. The clever combination of the functional DNA hairpin and the novel device achieved a ratiometric electrochemical aptasensor, which could be used as a simple, sensitive high repeatability and selective platform for target protein detection. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:158 / 163
页数:6
相关论文
共 58 条
[1]   Dual Nanoparticle Amplified Surface Plasmon Resonance Detection of Thrombin at Subattomolar Concentrations [J].
Baek, Seung Hee ;
Wark, Alastair W. ;
Lee, Hye Jin .
ANALYTICAL CHEMISTRY, 2014, 86 (19) :9824-9829
[2]  
Baker BR, 2006, J AM CHEM SOC, V128, P3138, DOI 10.1021/ja056957p
[3]   A fluorescent aptasensor based on DNA-scaffolded silver-nanocluster for ochratoxin A detection [J].
Chen, Jinghua ;
Zhang, Xi ;
Cai, Shuxian ;
Wu, Dongzhi ;
Chen, Mei ;
Wang, Shihua ;
Zhang, Jing .
BIOSENSORS & BIOELECTRONICS, 2014, 57 :226-231
[4]   Design and Biosensing of Mg2+-Dependent DNAzyme-Triggered Ratiometric Electrochemiluminescence [J].
Cheng, Yan ;
Huang, Yin ;
Lei, Jianping ;
Zhang, Lei ;
Ju, Huangxian .
ANALYTICAL CHEMISTRY, 2014, 86 (10) :5158-5163
[5]   An off-on-off electrochemiluminescence approach for ultrasensitive detection of thrombin [J].
Deng, Li ;
Du, Ying ;
Xu, Jing-Juan ;
Chen, Hong-Yuan .
BIOSENSORS & BIOELECTRONICS, 2014, 59 :58-63
[6]   Label-Free Voltammetric Aptasensor for the Sensitive Detection of Microcystin-LR Using Graphene-Modified Electrodes [J].
Eissa, Shimaa ;
Ng, Andy ;
Siaj, Mohamed ;
Zourob, Mohammed .
ANALYTICAL CHEMISTRY, 2014, 86 (15) :7551-7557
[7]   Target induced dissociation (TID) strategy for the development of electrochemical aptamer-based biosensor [J].
Han, Kun ;
Chen, Lin ;
Lin, Zhaosheng ;
Li, Genxi .
ELECTROCHEMISTRY COMMUNICATIONS, 2009, 11 (01) :157-160
[8]   Fluorescence Aptameric Sensor for Strand Displacement Amplification Detection of Cocaine [J].
He, Jing-Lin ;
Wu, Zai-Sheng ;
Zhou, Hui ;
Wang, Hong-Qi ;
Jiang, Jian-Hui ;
Shen, Guo-Li ;
Yu, Ru-Qin .
ANALYTICAL CHEMISTRY, 2010, 82 (04) :1358-1364
[9]   Ultrasensitive detection of thrombin using surface plasmon resonance and quartz crystal microbalance sensors by aptamer-based rolling circle amplification and nanoparticle signal enhancement [J].
He, Peng ;
Liu, Lijun ;
Qiao, Wenping ;
Zhang, Shusheng .
CHEMICAL COMMUNICATIONS, 2014, 50 (12) :1481-1484
[10]   Rolling Chain Amplification Based Signal-Enhanced Electrochemical Aptasensor for Ultrasensitive Detection of Ochratoxin A [J].
Huang, Lin ;
Wu, Jingjing ;
Zheng, Lei ;
Qian, Haisheng ;
Xue, Feng ;
Wu, Yucheng ;
Pan, Daodong ;
Adeloju, Samuel B. ;
Chen, Wei .
ANALYTICAL CHEMISTRY, 2013, 85 (22) :10842-10849