Cryopreservation in Tissue Engineering: Challenges & Prospects

被引：2

作者：

Sarangi, S. K. ^{[1
]}

Pramanik, K. ^{[2
]}

机构：

[1] Natl Inst Technol, Dept Mech Engn, Rourkela, India

[2] Natl Inst Technol, Dept Biotechnol & Med Engn, Rourkela, India

来源：

2010 ADVANCED TECHNOLOGIES FOR ENHANCING QUALITY OF LIFE (AT-EQUAL) | 2010年

关键词：

D O I：

10.1109/ATEQUAL.2010.8

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This paper describes the prospects and challenges of cryopreservation of tissue and tissue engineered products for their application to tissue repair and regeneration. The various aspects of cryopreservation include optimization of cell specific freezing parameters such as cooling rate, type and concentration of cryoprotective agents (CPA), media, treatment methods, warming and thawing for the preservation of tissue and tissue engineered products. The key issues related to the cryopreservation of tissue engineered constructs have been addressed explaining the occurrence of physicochemical and biophysical phenomena during cryopreservation, heat and mass transfer limitation and effect of large size of constructs on toxicity and cell viability. It has been described the effect of ice-forming and ice-free cryopreservation on cell viability, control of ice crystal formation by anti-freezing agent and cryopreservation equipments. A brief of ongoing research on cryopreservation of MNCs containing hematopoietic stems cells isolated from cord blood has also been presented.

引用

页码：114 / 120

页数：7

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