Isolation, analysis and marker utility of novel miniature inverted repeat transposable elements from the barley genome

被引:20
作者
Lyons, Maura [1 ]
Cardle, Linda [2 ]
Rostoks, Nils [2 ]
Waugh, Robbie [1 ]
Flavell, Andrew J. [1 ]
机构
[1] Univ Dundee SCRI, Div Plant Sci, Dundee DD2 5DA, Scotland
[2] Scottish Crop Res Inst, Genet Programme, Dundee DD2 5DA, Scotland
基金
英国生物技术与生命科学研究理事会;
关键词
barley; genetic diversity; transposable element; SSAP; MITE;
D O I
10.1007/s00438-008-0363-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Four previously undescribed families of miniature inverted repeat transposable elements (MITEs) were isolated by searching barley genomic DNA using structure-based criteria. Putative MITEs were confirmed by PCR to determine their insertional polymorphism in a panel of diverse barley germplasm. Copy numbers for all these familes are somewhat low (less than 1,000 copies per family per haploid genome). In contrast to previous studies, a higher proportion of insertions of the new MITEs are found within known transposable elements (27%) than are associated with genes (15%). Preliminary studies were conducted on two of the new MITE families to test their utility as molecular markers. Insertional polymorphism levels for both the families are high and diversity trees produced by both the families are similar and congruent with known relationships among the germplasm studied, suggesting that both the MITE families are useful markers of barley genetic diversity.
引用
收藏
页码:275 / 285
页数:11
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