Expanding the catalytic repertoire of nucleic acid catalysts:: Simultaneous incorporation of two modified deoxyribonucleoside triphosphates bearing ammonium and imidazolyl functionalities.

被引：68
作者：
Perrin, DM [1 ]
Garestier, T [1 ]
Hélène, C [1 ]
机构：
[1] Museum Natl Hist Nat, Biophys Lab, F-75005 Paris, France
来源：
NUCLEOSIDES & NUCLEOTIDES | 1999年 / 18卷 / 03期
关键词：
D O I：
10.1080/15257779908043083
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two nucleoside triphosphates, a pyrimidine modified with an ammonium functionality and a purine modified with an imidazolyl functionality are compatible with all conditions for a combinatorial selection of nucleic-acid catalysts. We believe that this work is the first to demonstrate the potential for using not one but two modified nucleotides in tandem. The potential for an enriched catalytic repertoire is envisioned.
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页码：377 / 391
页数：15
相关论文
共 43 条
  • [1] Breaker R R, 1994, Chem Biol, V1, P223, DOI 10.1016/1074-5521(94)90014-0
  • [2] DNA enzymes
    Breaker, RR
    [J]. NATURE BIOTECHNOLOGY, 1997, 15 (05) : 427 - 431
  • [3] ISOLATION OF A RIBOZYME WITH 5'-5' LIGASE ACTIVITY
    CHAPMAN, KB
    SZOSTAK, JW
    [J]. CHEMISTRY & BIOLOGY, 1995, 2 (05): : 325 - 333
  • [4] SEQUENCE-SPECIFIC SCISSION OF DNA BY THE CHEMICAL NUCLEASE ACTIVITY OF 1,10-PHENANTHROLINE-COPPER(I) TARGETED BY RNA
    CHEN, CHB
    GORIN, MB
    SIGMAN, DS
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (09) : 4206 - 4210
  • [5] SYNTHESIS OF 2'-DEOXYURIDINE NUCLEOSIDES WITH APPENDED 5-POSITION CARBONYL CROSS-LINKING GROUPS
    CROUCH, GJ
    EATON, BE
    [J]. NUCLEOSIDES & NUCLEOTIDES, 1994, 13 (04): : 939 - 944
  • [6] TRIPLE-HELIX SPECIFIC LIGANDS STABILIZE H-DNA CONFORMATION
    DUVALVALENTIN, G
    DEBIZEMONT, T
    TAKASUGI, M
    MERGNY, JL
    BISAGNI, E
    HELENE, C
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1995, 247 (05) : 847 - 858
  • [7] EKLAND EH, 1993, NATURE, V383, P373
  • [8] SELECTION INVITRO OF SINGLE-STRANDED-DNA MOLECULES THAT FOLD INTO SPECIFIC LIGAND-BINDING STRUCTURES
    ELLINGTON, AD
    SZOSTAK, JW
    [J]. NATURE, 1992, 355 (6363) : 850 - 852
  • [9] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS
    ELLINGTON, AD
    SZOSTAK, JW
    [J]. NATURE, 1990, 346 (6287) : 818 - 822
  • [10] FERSHT A, 1977, ENZYME STRUCTURE MEC, P426
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