Overexpression of Cyclin L2 Inhibits Growth and Enhances Chemosensitivity in Human Gastric Cancer Cells

被引：12
作者：
Li, Hong-Li [1 ]
Huang, Ding-Zhi [1 ]
Deng, Ting [1 ]
Zhou, Li-Kun [1 ]
Wang, Xia [1 ]
Bai, Ming [1 ]
Ba, Yi [1 ]
机构：
[1] Tianjin Med Univ, Dept Gastrointestinal Oncol, Tianjin Key Lab Canc Prevent & Therapy, Canc Inst & Hosp, Tianjin, Peoples R China
来源：
ASIAN PACIFIC JOURNAL OF CANCER PREVENTION | 2012年 / 13卷 / 04期
关键词：
Cyclin L2; gastric cancer; apoptosis; cell cycle; chemosensitivity; APOPTOSIS; CHEMOTHERAPY; CISPLATIN; PATHWAYS; THERAPY; ARREST;
D O I：
10.7314/APJCP.2012.13.4.1425
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Cyclin L2 is a novel member of the cyclin family, recently implicated in the regulation of cell cycle progression and/or transcriptional regulation. The present study was undertaken to investigate the effects of overexpression on tumor cell growth and chemosensitivity in human gastric cells in vitro. Cyclin L2 was transfected into human gastric cancer cell line BCG823 and expressed with a mammalian expression vector pcDNA3.1. The effects and mechanisms of cyclin L2 on cell growth, cell cycling and apoptosis were studied. Compared to control vectors, overexpression of cyclin L2 inhibited the growth of BCG823 cells and enhance their chemosensitivity to fluorouracil, docetaxel and cisplatin. The anti-proliferative effects of cyclin L2 could be due to G0/G1 arrest and apoptosis. Cyclin L2 induced G0/G1 arrest and apoptosis involved upregulation of caspase-3 and down regulation Bcl-2 and survivin. The results indicated that overexpression of cyclin L2 protein may promote efficient growth inhibition and enhance chemosensitivity to chemotherapeutic agents in human gastric cancer cells by inducing G0/G1 cell cycle arrest and apoptosis.
引用
收藏
页码：1425 / 1430
页数：6
相关论文
共 21 条
[1]   Clinical benefit with docetaxel plus fluorouracil and cisplatin compared with cisplatin and fluorouracil in a phase III trial of advanced gastric or gastroesophageal cancer adenocarcinoma: The V-325 study group [J].
Ajani, Jaffer A. ;
Moiseyenko, Vladimir M. ;
Tjulandin, Sergei ;
Majlis, Alejandro ;
Constenla, Manuel ;
Boni, Corrado ;
Rodrigues, Adriano ;
Fodor, Miguel ;
Chao, Yee ;
Voznyi, Edouard ;
Marabotti, Cindy ;
Van Cutsem, Eric .
JOURNAL OF CLINICAL ONCOLOGY, 2007, 25 (22) :3205-3209
[2]  
Alenzi FQ, 2010, ASIAN PAC J CANCER P, V11, P271
[3]  
[Anonymous], CA CANC J CLIN, DOI DOI 10.3322/CAAC.20107
[4]  
Bang YJ, 2010, LANCET, V376, P1302
[5]   Involvement of both intrinsic and extrinsic pathways in IFN-γ-induced apoptosis that are enhanced with cisplatin [J].
Barton, C ;
Davies, D ;
Balkwill, F ;
Burke, F .
EUROPEAN JOURNAL OF CANCER, 2005, 41 (10) :1474-1486
[6]   Characterization of cyclin L2, a novel cyclin with an arginine/serine-rich domain -: Phosphorylation by DYRK1A and colocalization with splicing factors [J].
de Graaf, K ;
Hekerman, P ;
Spelten, O ;
Herrmann, A ;
Packman, LC ;
Büssow, K ;
Müller-Newen, G ;
Becker, W .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (06) :4612-4624
[7]   Hedgehog regulates cell growth and proliferation by inducing cyclin D and cyclin E [J].
Duman-Scheel, M ;
Weng, L ;
Xin, SJ ;
Du, W .
NATURE, 2002, 417 (6886) :299-304
[8]   Cyclin B1 and other cyclins as tumor antigens in immunosurveillance and immunotherapy of cancer [J].
Egloff, AM ;
Vella, LA ;
Finn, OJ .
CANCER RESEARCH, 2006, 66 (01) :6-9
[9]   Enhanced antitumor therapy by inhibition of p21waf1 in human malignant mesothelioma [J].
Lazzarini, Raffaella ;
Moretti, Simona ;
Orecchia, Sara ;
Betta, Pier-Giacomo ;
Procopio, Antonio ;
Catalano, Alfonso .
CLINICAL CANCER RESEARCH, 2008, 14 (16) :5099-5107
[10]   Overexpression of cyclin L2 induces apoptosis and cell-cycle arrest in human lung cancer cells [J].
Li Hong-li ;
Wang Tong-shan ;
Li Xiao-yu ;
Li Nan ;
Huang Ding-zhi ;
Chen Qi ;
Ba Yi .
CHINESE MEDICAL JOURNAL, 2007, 120 (10) :905-909
123